[galaxy-user] Tophat results

2012-09-10 Thread Xiefan Fang
combine the FASTQ files for each biological sample and do the statistics again? At last, does anyone know how to convert a long list of zebrafish genes (500-1000 genes) to human or mammalian orthologs? Thank you for your replies, Xiefan Fang University of Florida _

[galaxy-user] SNP finding

2012-10-21 Thread Xiefan Fang
Dear galaxy users, We have done deep sequencing on some known genomic loci using Hiseq2000. I have already mapped the reads to the reference sequences by using Galaxy. In the next step, I want to find SNPs and calculate the SNP percentage within the reads. There are 500,000 to 1,000,000 reads p

[galaxy-user] SNP finding

2012-10-21 Thread Xiefan Fang
Dear galaxy users, We have done deep sequencing on some known genomic loci using Hiseq2000. I have already mapped the reads to the reference sequences by using Galaxy. In the next step, I want to find SNPs and calculate the SNP percentage within the reads. There are 500,000 to 1,000,000 reads p