Re: [galaxy-user] BED to BAM conversion in Galaxy

2011-10-04 Thread Jennifer Jackson
Hello, The last line of the report you sent suggests that the file has format problems. I read through your other email and noticed that a few steps were inserted, perhaps because they were needed. However, the line noted here is not in BED format: On 10/4/11 12:06 PM, shamsher jagat wrote:

Re: [galaxy-user] BED to BAM conversion in Galaxy

2011-09-29 Thread shamsher jagat
Thanks Jen, My problem is I have ChIP-seq data where I have one Bed file with coordinates- chr1 724027 724226 61PDWAAXX100706:4:19:6952:18071 - Then there is wig file.? Is it possible that thsi data can be analyzed in Galaxy/ Cistrome. I tried to use Cistrome which gav eme

Re: [galaxy-user] BED to BAM conversion in Galaxy

2011-09-28 Thread Jennifer Jackson
Hello, It is possible to go from SAM/BAM to BED, but not the reverse. SAM/BAM files contain the actual sequence data associated with the original aligned read. BED files only have the reference genome location of the alignment (no read sequence). It is possible to extract genomic sequence

[galaxy-user] BED to BAM conversion in Galaxy

2011-09-22 Thread shamsher jagat
Is it possible to use some tool in Galaxy to convert BED file to Bam/ sam file. In other word do we have Bed tools or other option in Galaxy Thanks ___ The Galaxy User list should be used for the discussion of Galaxy analysis and other