Re: [galaxy-user] Can I convert paired-end datasets into single end ones?

2012-08-15 Thread Jennifer Jackson
Jianguang, This is not recommended. The value of the paired relationships would be lost. Using an estimated Mean Inner Distance is a much better solution. This is keeping in mind that testing different values may be necessary to obtain the optimal results for any dataset. Your situation abou

[galaxy-user] Can I convert paired-end datasets into single end ones?

2012-08-15 Thread Du, Jianguang
Dear All, I have some paired-end datasets to be analyzed, but I am not sure about their Mean Inner Distance between Mate Pairs. Can I convert these paired-end datasets into single-end ones and use them as single-end dataset as follows? 1) Use the tool "Manipulate FASTQ" to convert the sequence