Hi Gema,
Quick comment. If you do have the QUAL file to begin with, I would
recommend doing the combine first, then barcode splitter(which can do
fastq too), then bowtie. This way you have the benefits of mapping
using the real quality score.
--Carlos
On Wed, Apr 3, 2013 at 11:07 AM, Daniel Bla
Hi Gema,
For example, see the "Combine FASTA and QUAL into FASTQ" tool for creating a
FASTQ file from FASTA data; when quality data is not provided, fake score
values will be used.
Thanks for using Galaxy,
Dan
On Apr 3, 2013, at 10:42 AM, Peter Cock wrote:
>
>
> On Wed, Apr 3, 2013 at 3:4
On Wed, Apr 3, 2013 at 3:40 PM, Gema Sanz Santos wrote:
> Hello,
>
> I'm trying to change the format to the output files from Barcode splitter
> from FASTA to FASTAQ so I can use them in Bowtie for Illumina. I've read
> that it can be done through the edit attributes, I go to datatype and
> selec
Hello,
I'm trying to change the format to the output files from Barcode splitter
from FASTA to FASTAQ so I can use them in Bowtie for Illumina. I've read
that it can be done through the edit attributes, I go to datatype and select
fastaq, save and then go to convert format and press convert but th
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