Re: [galaxy-user] Cuffdiff statistical calculations are inconsistent?

2013-03-15 Thread Jeremy Goecks
The header of the Cuffdiff tool page says it is version 0.0.5 This version is the Galaxy tool wrapper version, not the tool version. (Yes, this is a usability issue.) You can find the tool version in the dataset's information panel by clicking on the 'i' icon. Is there a way, or setting, on

[galaxy-user] Cuffdiff statistical calculations are inconsistent?

2013-03-13 Thread Jenna Smith
Hi, I'll preface my concern by saying that I'm a novice to Cufflinks. Back in September, I performed a Cuffdiff analysis comparing a wild-type and mutant condition. The analysis returned ~800 transcripts differentially regulated between the two with statistical significance. Recently, I've

Re: [galaxy-user] Cuffdiff statistical calculations are inconsistent?

2013-03-13 Thread Mohammad Heydarian
We are having the exact same issue, on the main server and our (recent) cloud instances. Were some of the hidden Cuffdiff parameters modified since fall 2012? Cheers, Mo Heydarian On Mar 13, 2013 11:02 AM, Jenna Smith jes...@case.edu wrote: Hi, I'll preface my concern by saying that I'm a

Re: [galaxy-user] Cuffdiff statistical calculations are inconsistent?

2013-03-13 Thread Jeremy Goecks
This is likely due to the upgrade from Cufflinks 1.3.x to Cufflinks 2.0.x; Cufflinks 2.0 introduced a new algorithm for Cuffdiff in particular. You can read about these changes on the website: http://cufflinks.cbcb.umd.edu/ (and there's a manuscript describing the changes as well). You might