Hello,
The FASTQ Joiner tool is currently being updated to work with the newer
sequence Id format. The progress of this change can be tracked here:
https://bitbucket.org/galaxy/galaxy-central/issue/677/update-joiner-tool-to-work-with-casava-18
Meanwhile, quality filtering can be done on each file independently,
then to synch up the two files (in case sequences are lost in one of the
files for quality reasons), a work-around method is:
- NGS: QC and manipulation - FASTQ to Tabular on both files
- Join, Subtract and Group - Join two Datasets on c1 from both files
Keep lines of first input that do not join with second input: as yes
Keep lines of first input that are incomplete: as no
Fill empty columns: as no
- NGS: QC and manipulation - Tabular to FASTQ run twice
Recreate both FASTQ files from the same tabular file.
The same sequence identifier column will be used in
both runs.
Hopefully this helps until we have the the regular FASTQ manipulation
tools updated,
Jen
Galaxy team
On 4/3/12 12:44 PM, meganathan pr wrote:
Hi,
I have HiSeq2000 paired end sequence data in two separate FASTQ
files. I need to filter the low quality scored sequences from my data to
have a good assembly. So I decided to join the PE reads and then filter
the low quality sequences in Galaxy.
To do this first I groomed the data using FASTQ groomer where I kept
Sanger as Input FASTQ quality scores type. Then I tried to join the PE
sequences using FASTQ joiner. However the FASTQ joiner did not join the
PE sequences but only shown the failure Info as follows
*FASTQ joiner on data 8 and data 9*
0 bytes
format: fastqsanger, database: ?
https://main.g2.bx.psu.edu/datasets/d08dd42f0e2ed22b/edit
Info: There were 400 known sequence reads not utilized.
Joined 0 of 400 read pairs (0.00%).
I am a new user and I have no idea where I am going wrong. Please
suggest me how to overcome this problem.
Thanks.
--
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