Forgive the forwarded email. Not used to working from an iPad. > On 2011-09-29, at 7:58 PM, Peter Cock <p.j.a.c...@googlemail.com> wrote: > >> On Thu, Sep 29, 2011 at 7:49 PM, Cecilia Tamborindeguy >> <ctamborinde...@ag.tamu.edu> wrote: >>> Hello, >>> >>> I would like to know if Galaxy can do de novo assembly without a reference >>> genome. >>> >>> Thanks. >>> >>> Cecilia >> >> Are you trying to use the Public Galaxy or a local install? There >> are several assemblers with Galaxy Wrappers on the Galaxy >> ToolShed (e.g. Roche "Newbler", and MIRA 3) which you could >> add to your own local Galaxy if you have one. >> >> However, do novo genome assembly can be very computationally >> demanding, so not many Galaxy Instances will want to offer it. >> >> Peter > > I would like to echo Peter's advice. (Again, that's twice in 5 min from 2 > different lists. I promise you I'm not stalking you, Peter). > > Genome assembly is a bit of a dedicated domain with respect to expertise and > time. If possible, if you are assembling a lot of genome data, you really > should set yourself up properly with a multiple-CPU unix box with a lot of > RAM and dedicate it to assembly. Install MIRA, Newbler, Velvet, AMOS, > samtools, bamtools, bedtools, Staden, phred/phrap/consed on it, and you can > assemble and interconvert data to your heart's content. > > Galaxy is a wonderful and useful service but assembling genomes does require > dedicated power and expertise, and preferably in house. I just forked out for > a 64 CPU processor with 1 TB RAM bc we assemble lots of genomes. You don't > have to go that far but a 3-4 quad processor box with 128 GB RAM and 1 TB > disk should be on your mind. > > John
___________________________________________________________ The Galaxy User list should be used for the discussion of Galaxy analysis and other features on the public server at usegalaxy.org. Please keep all replies on the list by using "reply all" in your mail client. For discussion of local Galaxy instances and the Galaxy source code, please use the Galaxy Development list:
http://lists.bx.psu.edu/listinfo/galaxy-dev To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/
