Re: [galaxy-user] GATK Not running

2012-12-13 Thread Farooq,Umar (res)
: total time 0.06 From: Daniel Blankenberg [d...@bx.psu.edu] Sent: Wednesday, December 12, 2012 4:29 PM To: Farooq,Umar (res) Cc: galaxy-user@lists.bx.psu.edu Subject: Re: [galaxy-user] GATK Not running Hi Umar, Can you click the eye icon to view the contents

Re: [galaxy-user] GATK Not running

2012-12-12 Thread Daniel Blankenberg
Hi Umar, Can you click the eye icon to view the contents of the 'log' dataset for the GATK run. The end of the log should have the actual error encountered (the text you provided is a bit of a red herring) Since you are using hg19, the most likely cause for the error is that the reference

Re: [galaxy-user] GATK Not running

2012-12-12 Thread Daniel Blankenberg
Hi Philippe, The GATK wrappers provided with the Galaxy distribution are for GATK version 1.4. There is a set of 1.6/GATK-lite wrappers that has been developed by the team, but is not yet available. There may also be other options available in the Tool Shed that have been contributed by the

Re: [galaxy-user] GATK Not running

2012-12-12 Thread Daniel Blankenberg
Hi Joshua, Is this on the main public site? If so, can you share your history with me and I'll take a look? If this is on a local instance, can you provide additional information, such as the GATK version that you are using? Thanks for using Galaxy, Dan On Dec 11, 2012, at 5:34 PM, Joshua

[galaxy-user] GATK Not running

2012-12-11 Thread Farooq,Umar (res)
Hi, I am trying to incorporate GATK in my pipeline but not been able to make it work. I aligned my data with Hg 19 and then ran sam tool filter and then picard duplicate removal. I uploaded dbSNP and the reference FASTA file for Hg 19 in galaxy to run this pipeline. But for some reason GATK

Re: [galaxy-user] GATK Not running

2012-12-11 Thread Philipe Moncuquet
Hi, I have encountered the same kind of errors. When I update the loc files link to GATK, some of the tools display the reference genomes I added and some not. It seems that the galaxy wrapper for GATK 1.6 is not very functional. GATK don't really care because they are not supporting it any more,

Re: [galaxy-user] GATK Not running

2012-12-11 Thread Joshua Orvis
I'm having some problems with GATK as well, but do have a functional pipeline that uses the following GATK tools in Galaxy: - Realigner Target Creator - Indel Realigner - Unified Genotyper - Variant Filtration The main problem I'm having with them is that it seems I need to run the fasta/fastq

Re: [galaxy-user] GATK Not running

2012-12-11 Thread Farooq,Umar (res)
cannot use GATK in galaxy. From: Joshua Orvis [jor...@gmail.com] Sent: Tuesday, December 11, 2012 5:34 PM To: Philipe Moncuquet Cc: Farooq,Umar (res); galaxy-user@lists.bx.psu.edu Subject: Re: [galaxy-user] GATK Not running I'm having some problems