Good Morning,
I confirmed with Illumina that my reads are in Sanger FASTQ format and used
edit attributes to change them to fastqsanger. Then I joined the forward
and reverse and ran the FastQ Joiner and the FastQC on the joined data
file. I'm getting the following error:
## odpath=None: No
Hello Lindsey,
Would you please send in a bug report from the FastQC error dataset that
resulted from the run where you included running the Groomer tool first?
This will help us to track down the problem.
Please be sure to leave all datasets in your history for this run
undeleted and in
Hello Lindsey,
Yes, you have this correct. The general path would be to:
- join forward and reverse data per run
- run FASTQ Groomer FastQC
(note: if your data is already in Sanger FASTQ format with Phred+33
quality scaled
values, the datatype '.fastqsanger' can be directly assigned
I am trying to do RNAseq analysis on Paired end data from the Hiseq2000. I
have about 50 files for each sample (25 forward and 25 reverse - although
each sample has a different number of files).
I think that I need to:
-convert them into FASTQ sanger format using the FASTSQ groomer tool
-check
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