Re: [galaxy-user] Remove unpaired reads from quality-filtered pared-end fastq files.

Hi Hiroki, This question has come up before, and the best advice our team has to offer is that in most cases, filtering the data this way is unnecessary. Still - there are a few methods to do this, but they are tedious to do - one is to basically covert everything to tabular format, extract

Re: [galaxy-user] Remove unpaired reads from quality-filtered pared-end fastq files.

Not the most convenient solution, but what I normally do in this situation is to combine the two files, filter then split again. There are tools for combining and splitting paired fastq files in Galaxy. Hope it helps, Carlos On Jan 8, 2013 12:55 AM, 柴田 弘紀 hshib...@gen.kyushu-u.ac.jp wrote: Hi

[galaxy-user] Remove unpaired reads from quality-filtered pared-end fastq files.

Hi there, I obtained two fastq files from GA paired end run. I filtered each file by quality using fastq tool kit. Then some forward reads may be removed by low quality whereas the reverse counterparts are OK to be remained on the other file, or vice versa. I want to remove those unpaired