Hi Dr. Linney,
The values for FPKM are relative to each experiment: the set of
transcripts/genes assembled and evaluated, the normalization methods
used. For example, when different samples are input, a different set of
isoforms per gene can result from the assembly.
The manual, FAQ, and 'ho
Hello,
I am sure this has come up before and maybe I missed the answer.
If in using cuffdiff I run a sample 1(3 repeats) vs a sample 2 and then
run the same sample 1(3 repeats) against a sample 3 or a sample 4, I get
different value for fpkm from sample 1 each time.
Is there something going wron
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