Hello,
The Galaxy instance for LEfSe is run by another group. If you haven't
done so already, it might be best to contact them directly to see what
their suggestions are. The contact link is on the home page, in the
middle pane, last sentence:
http://huttenhower.org/galaxy/
Hopefully they will be able to help you transform the data into the
correct format,
Best,
Jen
Galaxy team
On 10/20/11 11:26 AM, Klaudyna Borewicz wrote:
Hi,
I would like to use Galaxy to run LEfSe, but I don't know how to get
the data into tabular format that is required
(http://huttenhower.org/galaxy/tool_runner?tool_id=LEfSe_for). My data
is 454 fasta files that I was analyzing with RDP to get the
classification. It works fine, I get .txt file that i can load to
Galaxy, it looks like this:
norank Root37646
unclassified_Root 9
domain Bacteria37637
unclassified_Bacteria 5998
phylum OD1 0
unclassified_OD10
genus OD1_genera_incertae_sedis 0
phylum BRC10
unclassified_BRC1 0
genus BRC1_genera_incertae_sedis 0
phylum Deferribacteres 0
unclassified_"Deferribacteres"0
class Deferribacteres 0
unclassified_Deferribacteres0
order Deferribacterales 0
unclassified_Deferribacterales 0
family Deferribacterales_incertae_sedis0
unclassified_Deferribacterales_incertae_sedis 0
genus Caldithrix 0
family Deferribacteraceae 0
unclassified_Deferribacteraceae 0
genus Calditerrivibrio0
genus Mucispirillum 0
.
but i need to have the labels in a hierarchical organization and I
cannot find the way to get it to work. Please let me know if you have
any suggestions, or maybe RDP is just not the way to go.
Thank you and hope to hear from you soon,
Klaudyna
--
Jennifer Jackson
http://usegalaxy.org
http://galaxyproject.org/wiki/Support
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