Re: [galaxy-user] hg19 reference gnome for Tophat2

2013-02-12 Thread Sachit Adhikari
Also, do I have make all the reference files executable?

On Wed, Feb 13, 2013 at 6:39 AM, Sachit Adhikari <
sachit.techner...@gmail.com> wrote:

> I downloaded the entire directory of UCSC for the reference gnome of
> Tophat2. It turns out that the Tophat2 and Bowtie2 uses the same reference
> gnome. I found a directory: Homo_sapiens/UCSC/hg19/Sequence/Bowtie2Index
>
> with the files:
>
> genome.1.bt2  genome.2.bt2  genome.3.bt2  genome.4.bt2  genome.rev.1.bt2
>  genome.rev.2.bt2
>
>
> While adding the reference gnome, I need to edit bowtie2_indices.loc
>
> Shall I replace:
>
> /orig/path/hg19hg19hg19
>  /depot/data2/galaxy/bowtie2/hg19/hg19
>
> with
>
> hg19   hg19hg19   Homo_sapiens/UCSC/hg19/Sequence/Bowtie2Index
>
>
> Thanks,
>
> Sachit
>
___
The Galaxy User list should be used for the discussion of
Galaxy analysis and other features on the public server
at usegalaxy.org.  Please keep all replies on the list by
using "reply all" in your mail client.  For discussion of
local Galaxy instances and the Galaxy source code, please
use the Galaxy Development list:

  http://lists.bx.psu.edu/listinfo/galaxy-dev

To manage your subscriptions to this and other Galaxy lists,
please use the interface at:

  http://lists.bx.psu.edu/

[galaxy-user] hg19 reference gnome for Tophat2

2013-02-12 Thread Sachit Adhikari
I downloaded the entire directory of UCSC for the reference gnome of
Tophat2. It turns out that the Tophat2 and Bowtie2 uses the same reference
gnome. I found a directory: Homo_sapiens/UCSC/hg19/Sequence/Bowtie2Index

with the files:

genome.1.bt2  genome.2.bt2  genome.3.bt2  genome.4.bt2  genome.rev.1.bt2
 genome.rev.2.bt2


While adding the reference gnome, I need to edit bowtie2_indices.loc

Shall I replace:

/orig/path/hg19hg19hg19
 /depot/data2/galaxy/bowtie2/hg19/hg19

with

hg19   hg19hg19   Homo_sapiens/UCSC/hg19/Sequence/Bowtie2Index


Thanks,

Sachit
___
The Galaxy User list should be used for the discussion of
Galaxy analysis and other features on the public server
at usegalaxy.org.  Please keep all replies on the list by
using "reply all" in your mail client.  For discussion of
local Galaxy instances and the Galaxy source code, please
use the Galaxy Development list:

  http://lists.bx.psu.edu/listinfo/galaxy-dev

To manage your subscriptions to this and other Galaxy lists,
please use the interface at:

  http://lists.bx.psu.edu/