Re: [galaxy-user] run tophat in galaxy

2011-10-05 Thread Jeremy Goecks
taller so it will be easier to see? > > Thanks, > > > tao > > -Original Message- > From: Jennifer Jackson [mailto:j...@bx.psu.edu] > Sent: Tuesday, September 27, 2011 1:13 PM > To: Peng, Tao > Cc: galaxy-user > Subject: Re: [galaxy-user] run tophat in

Re: [galaxy-user] run tophat in galaxy

2011-10-04 Thread Peng, Tao
/transcript expression from Cuffdiff results. Thanks, tao -Original Message- From: Peng, Tao Sent: Friday, September 30, 2011 3:13 PM To: 'Jennifer Jackson' Cc: galaxy-user Subject: RE: [galaxy-user] run tophat in galaxy Thanks a lot. Please see the attached screen shot

Re: [galaxy-user] run tophat in galaxy

2011-09-30 Thread Peng, Tao
[mailto:j...@bx.psu.edu] Sent: Tuesday, September 27, 2011 1:13 PM To: Peng, Tao Cc: galaxy-user Subject: Re: [galaxy-user] run tophat in galaxy Hi Tao, Yes, the resulting SAM dataset can be converted to BAM and viewed in the GTB (Galaxy Track Browser). http://galaxyproject.org/wiki/Learn -> scroll

Re: [galaxy-user] run tophat in galaxy

2011-09-28 Thread Peng, Tao
PM To: galaxy-user Cc: Peng, Tao Subject: Re: [galaxy-user] run tophat in galaxy ===> Please use "Reply All" when responding to this email! <=== Hi Tao, I made an error in my prior reply, it is possible to guide assembly in TopHat. To do this, on the TopHat form, change "T

Re: [galaxy-user] run tophat in galaxy

2011-09-27 Thread Jennifer Jackson
r Jackson [mailto:j...@bx.psu.edu] Sent: Thursday, September 15, 2011 2:37 PM To: galaxy-user Cc: Peng, Tao Subject: Re: [galaxy-user] run tophat in galaxy ===> Please use "Reply All" when responding to this email!<=== Hi Tao, I made an error in my prior reply, it is possible to gu

Re: [galaxy-user] run tophat in galaxy

2011-09-16 Thread Jennifer Jackson
===> Please use "Reply All" when responding to this email! <=== Hello, Chromosome names will need to be modified once the file is imported, as explained in #5 of the FAQ: http://usegalaxy.org/u/jeremy/p/transcriptome-analysis-faq Hopefully this helps, Best, Jen Galaxy team On 9/15/11 8:22

Re: [galaxy-user] run tophat in galaxy

2011-09-15 Thread shamsher jagat
I have related question If I have to use Ensembl mouse GTF file (Mus_musculus.NCBIM37.64) Do I have to download and reformat it or Galaxy can take it from the source directly? Thanks On Sun, Aug 28, 2011 at 8:50 AM, Peng, Tao wrote: > ===> Please use "Reply All" when responding to this email!

Re: [galaxy-user] run tophat in galaxy

2011-09-15 Thread Jennifer Jackson
===> Please use "Reply All" when responding to this email! <=== Hi Tao, I made an error in my prior reply, it is possible to guide assembly in TopHat. To do this, on the TopHat form, change "TopHat settings to use:" from "Use Defaults" to "Full parameter list". In the expanded form: 1 - chan

[galaxy-user] run tophat in galaxy

2011-09-15 Thread Jennifer Jackson
===> Please use "Reply All" when responding to this email! <=== Hello Tao, Sorry for the delayed reply, your question did not post to the mailing list since the "to" was not _only_ to galaxy-user. Going forward, please leave off any "to" or "cc" to team members when asking a question. Send a

[galaxy-user] run tophat in galaxy

2011-08-29 Thread Peng, Tao
===> Please use "Reply All" when responding to this email! <=== Hi how can I specify a GTF gene annotation file when running tophat to guide the alignment to human genome? What is the best way to visualize the tophat results in the context of annotated human genome, i.e. RefSeq? Thanks, tao