Re: [galaxy-user] Cuffdiff question

2013-11-15 Thread clare Hardman
Hi Noa, Yes I did use Cufflinks so this sounds just like my problem. So how have you dealt with the problem? Best wishes, Clare On 14 Nov 2013, at 18:17, Noa Sher wrote: > Hi Clare > We just ran into a similar issue about a week ago and were debugging with the > authors of cuffdiff > Appar

Re: [galaxy-user] Cuffdiff question

2013-11-15 Thread Noa.sher
Dont use the - b parameter Sent from my iPhone; please excuse any brevity or typos! On Nov 15, 2013, at 2:51 PM, clare Hardman wrote: > Hi Noa, > > Yes I did use Cufflinks so this sounds just like my problem. So how have you > dealt with the problem? > > Best wishes, > > Clare > > > > On

Re: [galaxy-user] Cuffdiff question

2013-11-14 Thread Noa Sher
Hi Clare We just ran into a similar issue about a week ago and were debugging with the authors of cuffdiff Apparently there are issues with the -b parameter - were you using this in cufflinks? If yes - this may be the cause - we switched the order of the replicate

Re: [galaxy-user] Cuffdiff question about using an unspecified (?) database/build

2011-08-19 Thread David K Crossman
t; box for analyses where I provide the fasta and gtf file. Thanks, David -Original Message- From: Jennifer Jackson [mailto:j...@bx.psu.edu] Sent: Friday, August 19, 2011 9:20 AM To: David K Crossman Cc: galaxy-user (galaxy-user@lists.bx.psu.edu) Subject: Re: [galaxy-user] Cuffdiff question

Re: [galaxy-user] Cuffdiff question about using an unspecified (?) database/build

2011-08-19 Thread Jennifer Jackson
Hello David, This is a known bug. The correction is planned to be moved out onto the public Galaxy instance at the next update (within a week). Sorry for the current inconvenience, Best, Jen Galaxy team On 8/19/11 7:00 AM, David K Crossman wrote: Hello! I have an RNA-Seq project which con

Re: [galaxy-user] Cuffdiff Question

2011-06-28 Thread Kurinji Pandiyan
Thanks for the reply. I tried to use the script provided on a previous galaxy thread for adding the chr on to the gtf file on the mac terminal but I keep getting this error - awk: can't open file ensembl.gtf source line number 1 I am very new to using the terminal so please let me know if there

Re: [galaxy-user] Cuffdiff Question

2011-06-28 Thread Jeremy Goecks
> Thanks for the reply. I tried to use the script provided on a previous galaxy > thread for adding the chr on to the gtf file on the mac terminal but I keep > getting this error - > > awk: can't open file ensembl.gtf > source line number 1 > > I am very new to using the terminal so please l

Re: [galaxy-user] Cuffdiff Question

2011-06-28 Thread Jeremy Goecks
Hello Kurinji, > I was at your USC Galaxy seminar last week, which I found very helpful - > thank you! Glad to hear that you found the workshop helpful. As a reminder, please email questions about using Galaxy and its tools to the galaxy-user mailing list (which I've cc'd). You may get quicker