Re: [galaxy-user] Flagstat on BAM files

2011-04-05 Thread Jennifer Jackson
Hi Slim, The tool is working correctly as we have it implemented right now, but there is some room for improvement. We are adding some tuning to our near-term to do list. Thanks for the suggestion! Best, Jen Galaxy team On 4/5/11 9:31 AM, Slim Sassi wrote: Hello, I tried to use NGS: SAM To

Re: [galaxy-user] Flagstat on BAM files

2011-04-05 Thread Sean Davis
On Apr 5, 2011 1:05 PM, "Slim Sassi" wrote: > > Sean, > You are correct, I did use tophat. Can you or anyone suggest a program for BAM/SAM stats where the alignment was done with tophat > Slim, What stats do you want to capture? The output you gave for flagstats is correct for single-end topha

Re: [galaxy-user] Flagstat on BAM files

2011-04-05 Thread Slim Sassi
Sean, You are correct, I did use tophat. Can you or anyone suggest a program for BAM/SAM stats where the alignment was done with tophat Thanks Slim On Apr 5, 2011, at 12:51 PM, Sean Davis wrote: > Hi, Slim. > > My guess is that you used an aligner that outputs only aligned reads > (tophat, for

Re: [galaxy-user] Flagstat on BAM files

2011-04-05 Thread Sean Davis
Hi, Slim. My guess is that you used an aligner that outputs only aligned reads (tophat, for example) and that the input was single-ended. If that is the case, then what you see below is exactly as expected. If not, then you might need to be more specific about how you generated the BAM file. Se