Re: [galaxy-user] GFF not recognized in CUFFLINK

2012-09-27 Thread Qian Dong
Dear Jennifer, My .fasta reference genome is like this: 'gi|289546492|ref|NC_011420.2| Rhodospirillum centenum SW chromosome, complete genome' and in the SAM file generated by BOWTIE it says: @SQ SN:gi|289546492|ref|NC_011420.2|LN:4355543 So I think they are all the same as

Re: [galaxy-user] GFF not recognized in CUFFLINK

2012-09-26 Thread Jennifer Jackson
Hello, The first thing to double check is that the chromosome identifier is an exact match between the reference genome and the reference annotation. The GFF3 file is naming the chromosome NC_011420.2. The reference annotation chromosome should be named exactly the same way. Check this in

Re: [galaxy-user] GFF not recognized in CUFFLINK

2012-09-26 Thread Kenneth Auerbach
Hi, I'm getting this cuffmerge error on our local Galaxy instance. I assume it is because it doesn't have the hg19 reference genome. Can you please tell me how I would include into this Galaxy installation? Thank you.

Re: [galaxy-user] GFF not recognized in CUFFLINK - Re: adding new genome

2012-09-26 Thread Jennifer Jackson
Hello, Instructions for including a new genome indexed for tools, including how to download the same indexes used on the public Main Galaxy instance hosted at http://usegalaxy.org, are in these wikis: http://wiki.g2.bx.psu.edu/Admin/Data%20Integration