On Mon, Feb 28, 2011 at 9:28 PM, Yang Liu <ywl5...@psu.edu> wrote: > > Is there any tool in Galaxy for trimming the Barcode > in 454 sequencing data? Thanks. >
Hi, The Roche off instrument applications cover this (working directly with the SFF files). Try asking Edward Kkirton if he would update his "Suite of Newbler tools" wrappers in the Community Tool Shed to handle barcode trimming (and dividing SFF files by barcode). See http://community.g2.bx.psu.edu/ Alternatively, and maybe not quite what you need, I'm working on barcode/primer/adapter trimming for FASTA, FASTQ and SFF files - see this thread earlier this month on the Galaxy dev mailing list: http://lists.bx.psu.edu/pipermail/galaxy-dev/2011-February/004344.html Peter _______________________________________________ The Galaxy User list should be used for the discussion of Galaxy analysis and other features on the public server at usegalaxy.org. For discussion of local Galaxy instances and the Galaxy source code, please use the Galaxy Development list: http://lists.bx.psu.edu/listinfo/galaxy-dev To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/
