Hello,
The coordinates are interpreted in Galaxy as having a 0-based start.
This means that in order to determine the actual start genome position,
add 1. Not a stupid question - everyone has to learn this as they begin
to work with data types sourced originally from UCSC and associated
projects.
Depending on which tool you are using in the UCSC database, the
coordinates will be interpreted as 0-based or 1-based. What tools
outside of UCSC or Galaxy do with the coordinates can vary.
In general:
positional coordinates of format "chrA:NNN-NNNN" will be 1-based
BED/Interval format will be 0-based
More help is on the "Convert formats" tool descriptions (included in BED
format description). And, this link at UCSC has all of the details:
http://genomewiki.ucsc.edu/index.php/Coordinate_Transforms
Hopefully this helps!
Best,
Jen
Galaxy team
On 4/6/11 11:15 PM, Sean wrote:
Hi,
I have one stupid question. The coordinates of the region chr1 2351533 -
2351843 from UCSC (hg18) will retrieve 311 bases. However, when I use
Fetch Sequences from galaxy, it will only retrieves 310 bases.
Apparently, the first base of the 311 bases is missing from the Fetch
Sequences result because the ending bases are the same.
Does this mean that I need to modified the coordinates first and then
use the Fetch Sequences to get the correct sequence? I thought UCSC and
galaxy were both 0 base?
Thanks.
Sean
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___________________________________________________________
The Galaxy User list should be used for the discussion of
Galaxy analysis and other features on the public server
at usegalaxy.org. Please keep all replies on the list by
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