Jianguang,
If your input dataset(s) have a genome assigned ("database") and that
genome is local to Galaxy (I believe this is true for your data, mm9,
correct?), then using bias correction would be an advantageous option to
make use of according to our experience and the tool authors in the
Cufflinks manuals/protocols/publications.
That said, the choice of whether or not to use Bias correction would be
ultimately up to you (a test using and not using could help with this
decision). From what I know, it is generally considered best practice to
use it when available.
Custom genomes can also be used with bias correction, but require a
different way of setting options and database assignments. Using no
reference genome is also possible (and therefore no bias correction),
again with different settings. The tool form has general instructions
around this as does the RNA-seq FAQ under Shared Data -> Published Pages
and the Custom genome wiki. In addition, there are several prior posts
with Q/A on the topic on the mailing list (a search would bring up the
settings for others reading this post that are curious). Anything that
remains unclear we can help with (send a new Q to the list) and we can
adjust the wiki, etc. to be more specific if not covered already.
http://galaxyproject.org/search/mailinglists
Take care,
Jen
Galaxy team
On 8/27/12 12:03 PM, Du, Jianguang wrote:
Dear All,
I am looking for the differential splicing events between cell types.
Although I got a lot of helps from Jen and from protocols found online,
I am still not sure about some settings for Cufflink, Cuffmerge and
Cuffdiff.
1) For Cufflink:
There is a setting for Bias Correction. I made the setting as below:
Perform Bias Correction:Yes
Reference sequence data:Locally cached
Did I make the right settings?
2) For Cuffmerge:
As for whether use sequence data, I made the setting as below:
Use Sequence Data: Yes
Choose the source for the reference list: Locally cached
Did I make the right settings?
3) For Cuffdiff:
There is another choice whether perform Bias Correction, I made the
setting as below:
Perform Bias Correction:Yes
Reference sequence data:Locally cached
Did I make the right settings?
Thanks.
Jianguang
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Jennifer Jackson
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___________________________________________________________
The Galaxy User list should be used for the discussion of
Galaxy analysis and other features on the public server
at usegalaxy.org. Please keep all replies on the list by
using "reply all" in your mail client. For discussion of
local Galaxy instances and the Galaxy source code, please
use the Galaxy Development list:
http://lists.bx.psu.edu/listinfo/galaxy-dev
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