Jiwen, I wonder if you are thinking about the situation where you
could be discarding reads that are to short after trimming?. In that
case you could be getting the two files out of sync. If this is the
case, I think you do need to join the files first, do the trimming and
then take them apart agai
Hello Jiwen,
No, you do not need to join the files for the quality processing.
Hopefully this helps!
Best,
Jen
Galaxy team
On 4/9/12 9:14 AM, 杨继文 wrote:
Hi all,
I have paired end RNA-Seq reads ( Illumina Hiseq 2000) in seperate
files. Before mapping, I need to trim the reads.
My questions i
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