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On Jun 28, 2013, at 12:01 PM, "galaxy-user-requ...@lists.bx.psu.edu"
<galaxy-user-requ...@lists.bx.psu.edu> wrote:

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> Today's Topics:
>
>   1. Re: gft annotation files (Jennifer Jackson)
>   2. Re: Pause function please (Jennifer Jackson)
>   3. Problem with ftp server? (Katrien M. Devos)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Thu, 27 Jun 2013 11:12:38 -0700
> From: Jennifer Jackson <j...@bx.psu.edu>
> To: Vijay Kumar <nayaganv...@gmail.com>
> Cc: galaxy-u...@bx.psu.edu
> Subject: Re: [galaxy-user] gft annotation files
> Message-ID: <51cc8096.2030...@bx.psu.edu>
> Content-Type: text/plain; charset="iso-8859-1"; Format="flowed"
>
> Hello,
>
> The UCSC Table browser can be used to extract a GTF file formatted
> dataset. See the tool "Get Data -> UCSC Main". The output format is one
> of the choices at the bottom of the tool UCSC form and there is a user
> guide below that at the UCSC web site that details how-to for queries.
>
> Best,
>
> Jen
> Galaxy team
>
>
> On 6/27/13 4:35 AM, Vijay Kumar wrote:
>> Dear sir,
>> any one suggest me where i download reference annotation gft files for
>> zebra finch . i tried ucsc site, but i donot know exact place to get
>> gft files. thank u sir..........
>>
>>
>> ___________________________________________________________
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>
> --
> Jennifer Hillman-Jackson
> Galaxy Support and Training
> http://galaxyproject.org
>
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> ------------------------------
>
> Message: 2
> Date: Thu, 27 Jun 2013 11:30:25 -0700
> From: Jennifer Jackson <j...@bx.psu.edu>
> To: "Ann Holtz-Morris, M.S." <aholtzmor...@chori.org>
> Cc: "galaxy-user@lists.bx.psu.edu" <galaxy-user@lists.bx.psu.edu>
> Subject: Re: [galaxy-user] Pause function please
> Message-ID: <51cc84c1.6010...@bx.psu.edu>
> Content-Type: text/plain; charset="iso-8859-1"; Format="flowed"
>
> Hi Ann,
>
> A pause function that truly stops an alignment job, permits inspection
> of intermediate results, then resumes it, is probably not going to be
> implemented. But we'll send more feedback about that if I have it incorrect.
>
> The best advice I have to consider running a smaller sample of data
> through as a test set. It can be tricky to make truly "representative"
> test sets at first pass, but after this run completes, you could
> probably identify one by coordinates by choosing all of the sequences
> that fit within a certain "slices" of genomic regions that contain
> transcript models (or whatever feature you are examining) that are
> interesting (or difficult!). Then use that with subsequent runs if you
> want to tune parameters and have it run quicker. This is what I usually
> do - being sure to include some of the uglier regions. Even if I am
> never really able to solve them completely, at least I know what they
> will look like in the end. You don't want to train your parameters on
> all "ideal" cases. With lower quality data, this might be even more
> important since you've got a double challenge to manage - query and
> target. But that's real data!
>
> Good luck with your project,
>
> Jen
> Galaxy team
>
> On 6/26/13 2:33 PM, Ann Holtz-Morris, M.S. wrote:
>>
>> Hi,
>>
>> I read
>> https://www.google.com/url?q=http://dev.list.galaxyproject.org/Can-t-find-new-pause-resume-functionality-in-Dec-2012-Galaxy-td4657331.html&sa=U&ei=KFzLUaHFCaGcjALSq4GQCw&ved=0CAcQFjAA&client=internal-uds-cse&usg=AFQjCNHSziHrbCCmumvDDUtmAeW9RvLRdg
>> with interest.
>>
>> I would also like a Pause function as a toggle on/off so I can stop
>> look at the data being generated. I'm running a BWA alignment that's
>> been working now for almost 48 hours. It's a decent sized data set but
>> it had a very low quality second sequencing read. I've quality trimmed
>> and length filtered. It ended up being only ~50% of the # of reads in
>> Read1.
>>
>> I'd love to be able to pause it and see if the data set is  behaving
>> as expected.
>>
>> Thanks,
>>
>> Ann
>>
>> Ann Holtz-Morris
>>
>> SRA III
>>
>> de Jong Laboratory
>>
>> 510-428-3885 x 5715
>>
>> aholtzmor...@chori.org <mailto:aholtzmor...@chori.org>
>>
>> Shipping and Mailing Address
>>
>> Attention: Ann Holtz-Morris
>>
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>>
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>>
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>> ___________________________________________________________
>> The Galaxy User list should be used for the discussion of
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> --
> Jennifer Hillman-Jackson
> Galaxy Support and Training
> http://galaxyproject.org
>
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> ------------------------------
>
> Message: 3
> Date: Thu, 27 Jun 2013 20:33:35 -0400
> From: "Katrien M. Devos" <kde...@uga.edu>
> To: galaxy-u...@bx.psu.edu
> Subject: [galaxy-user] Problem with ftp server?
> Message-ID:
>    <CAMU-1xh1gWG9BK5GK0=w3o-hhdntn61qdqr6x+cewzesbbt...@mail.gmail.com>
> Content-Type: text/plain; charset="iso-8859-1"
>
> I successfully uploaded files to the main Galaxy server this morning using
> ftp.  However, any attempts to upload files since around noon have failed.
> I get a 'Could not connect to server' error when trying to connect to
> main.g2.bx.psu.edu uisng FileZilla.  Any suggestions?
> Thank you.
> Katrien
>
> --
> Katrien M. Devos
> Dept. of Crop and Soil Sciences & Dept. of Plant Biology
> University of Georgia
> Athens, GA 30602
> USA
> Tel: (706) 542-0925
> Fax: (706) 542-0914
> E-mail: kde...@uga.edu
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> End of galaxy-user Digest, Vol 84, Issue 27
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