I fixed it, Thanks. It was because I aligned to different genomes in BWA and GATK steps
2012/4/3 Lilach Friedman <lilac...@gmail.com> > Hi Galaxy Team, > I'm trying to use GATK DepthOfCoverage, and to give a BAM file of alined > results as input. > > > My steps were: > 1. BWA alignment > 2. SAM-to-BAM > 3. rmdup. > > when I'm trying to give 3 (rmdup results) as an input to GATK > DepthOfCoverage, an error appears: " > Sequences are not currently available for the specified build." > > What did I do wrong? > > > Many thanks! > Lilach >
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