Hello William,
The tools in "NGS: QC and manipulation", especially those in the
sub-section "AB-SOLiD data" can do the manipulations needed before
mapping. It may be helpful to view the screencast at
http://usegalaxy.org, center pane, quickie #9.
Hopefully this helps to get you started,
Best,
Jen
Galaxy team
On 7/28/11 2:29 PM, William Light wrote:
I am trying to use bowtie to assign reads to the s. Cerevisiae genome.
I have data from paired end SOLiD sequencing with two unique six base
pair barcodes. Can I use bowtie to make csfasta and qual files from my
mixed original data split by bar code? I know I can use the trim option
to remove the barcode, but how do I specify one only?
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Jennifer Jackson
http://usegalaxy.org
http://galaxyproject.org/Support
___________________________________________________________
The Galaxy User list should be used for the discussion of
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at usegalaxy.org. Please keep all replies on the list by
using "reply all" in your mail client. For discussion of
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