Any chance you can get cervix? Ectocervix has glycogen in it. Just a
suggestion.
Peggy A. Wenk, HTL(ASCP)SLS
Beaumont Hospital
Royal Oak, MI 48073
-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of Debbie Hogg
Sent: Friday, October 10, 2008 9:59 PM
To: Hi
Hello:
Does anyone have any spare liver blocks that they could donate? Our hospital
does not perform that procedure and we have reached a zero level. Please
contact me and I will send my hospitals address to you. I will gladly pay for
the blocks and for postage to my place of work (Ontario,
In addition, ruling is from Department of Health and Human Services (DHHS),
Center for Medicaid and Medicare Services (CMS), 42 CFR 493.1252(c)
http://edocket.access.gpo.gov/cfr_2006/octqtr/pdf/42cfr493.1253.pdf
So it's bigger than CAP. It's the US government.
Peggy A. Wenk, HTL(ASCP)SLS
Beaumont
Unfortunately, "Expiration Date" is required by FDA/IVD and European CE/IVD
mark. No one is going to touch that one due to liabilities to a laboratory of
re-validating "expired" reagents.
Tim Morken
Technical Support Manager
Lab Vision Products
Anatomical Pathology
ThermoFisher Scientific
I have antibodies that work great 10-20 years after their expiration
date. Once again, I propose that the manufactures eliminate the
"expiration date" and use a "stability guarantee date". If the antibody
is used after that date then the user would be responsible for
validating its performance.
At the labs I have worked at we cut routine tissue at 3um, lymph nodes and
kidney biopsies at 2um, bone marrow clots and biopsies at 1 um.
Hope this helps,
Shelly
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We spray with a 3% solution of acetic acid.
Laurie
-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of Leslie
Chen
Sent: Friday, October 10, 2008 1:31 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] histology ink washing off
Hello,
I am having a
Spray with or dip in a solution of 5% acetic acid (vinegar) and the ink
will set...
Have a good weekend!
Joyce
Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
Please note new phone and fax numbers
678-843-7376 - Phone
678-843-7831 - Fax
I am looking for a Histology Supervisor in Northern, NJ.? Candidate should have
HT/HTL (ASCP) with some supervisory experience.? Qualified candidates should
e-mail their resume to [EMAIL PROTECTED]
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Hello,
I am having a problem with histology tissue marking ink washing off specimens
in water, PFA, or 70% alcohol. This is before the tissue even gets processed
for paraffin embedding. Does anyone have any suggestions on how to keep the ink
from coming off? Thank you.
Leslie
___
I'm from Oklahoma, but live in Tucson, so I guess that make me an AZ Wildcat
now...but a true sooner at heart...AND the two coaches just happen to be
brothers, s that makes my son in law my rivalLOL...ya'll have a GR8T
weekend.
~Kim ~
~Don't let your life end before it begins~
It works as good as methacarn, which is much cheaper.
René J.
--- On Fri, 10/10/08, Michael J. Lyon, Ph.D. <[EMAIL PROTECTED]> wrote:
From: Michael J. Lyon, Ph.D. <[EMAIL PROTECTED]>
Subject: [Histonet] UMFIX
To: histonet@lists.utsouthwestern.edu
Date: Friday, October 10, 2008, 11:31 AM
Does any
If you would like to try a alternative to the "cryoembedder" you may be
interested in
trying our "Precision cryoembedding system". By embedding tissue face down in
freezing temperature "well bars" you adhere tissue perfectly flat to the well
floor.
Multiple tissue pieces can be precisely prepa
Hi Sharon,
I watched the Blue Angels practicing yesterday, just outside my lab window
here in Mission Bay! I hope they practice again today! They were
absolutely beautiful!
Have fun sailing tomorrow!
Jo Dee
~~Jo Dee Fish~~
Senior Research Technologist
The J. David Gladstone Institutes
Co-manag
Gene:
Dako and Biocare both sell the stable pepsin solution. It is easy to
prepare if you want to make it yourself.
I use this pepsin everyday. Here is the recipe that Dr. Brigati gave me.
Best Regards,
Jim Burchette
Brigati’s 0.25% Stable Pepsin Solution
Reagents
Pepsin, #P7012, Sigm
Hi everybody,
does anyone know a reliable marker for kidney glomerular and
tubulointerstitial endothelial cells in rats and mice? Which
antibodies do work??
In our hands only JG12 Ab works for rat glomerular but not
interstitial endothelium and VEGFR2 in mice the other way round.
Many tha
Histonetters!
Taking off on Kim Tournear's message of "OU Rocks!" to say one of the best
college games of the year if not so far this century will be played out in the
Cotton Bowl, Dallas tomorrow morning at 11:00AM CDT. It is between Oklahom #1
and Univ Texas #5 and will be a true grudge
Great opportunity for Histotechnician in brand new laboratory!
Gastrointestinal Associates of North Texas in Ft Worth , is looking for
a certified HT or HTL to run their newly constructed laboratory.
Candidate must be ASCP certified and meet CLIA-88 regulations to perform
gross dissection. Prior su
Although both are flammable and give off toxic fumes, toluene is slightly more
dangerous because it has a higher vapor pressure. The flash point of toluene
is 5 degrees C; the flash point of xylene is around 25 degrees C (Merck index;
Lewis & Sax say 38 degrees C).
-Original Message-
F
I will be out of the office starting 10-Oct-2008 and will not return until
14-Oct-2008.
During my absence contact Jim Nold about your anatomic path issues. I will
respond to messages when I return.
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The Leica CM 1100 doesn't let you adjust the angle of the block face. You
have to be flat. I'll admit it's a major drawback. I didn't even realize
it until you mentioned this. I just got off the phone with leica and they
don't have anything to replace the specimen holder. Looks like I'm just
s
OK,
Here's the Cryo Embedder info:
The Vendor is:
Belaire Instruments 1-973-912-8900
Contact is:
Sheila Skolnick ext 112
Price is:
$1695.00
When ordering, there is no cat #.
It's ordered as a "Complete Cryo-Embedder Package"
I've been doing Mohs for years and have used all of the old met
PLEASE TAKE ME OFF THE MAILING LIST FOR THE TIME BEING FOR I WILL BE OUT OF
TOWN FOR NEXT COUPLE OF MONTHS.
MY ADDRESS IS [EMAIL PROTECTED]
THANKS, ED.
_
Stay up to date on your PC, the Web, and your mobile phone with Windows Live
Does anyone have experience with UMFIX a methanol/polyethylene glycol
fixative sold by Sakura Tissue-Tek, Torrance, CA?
If so good, bad or indifferent?
Thanks
Michael J. Lyon, Ph.D.
Otolaryngology Research Lab
SUNY Upstate Medical University
750 East Adams Street
Syracuse, NY 13
Hi Histonetters!
I just wanted to do a quick post before we all get busy with our weekend
football games, festivals and all the other great things that we get to
do this time of year when the weather is great everywhere!!
I have some new positions that I am working on that I wanted to tell you
abou
I don't think you'll get much flaming on that statement Jacquie, just a
lot of agreement!
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596
-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL P
I agree, it is a very ridiculous rule, and I don't like having to
eliminate perfectly good antibodies or reagents. When you look at the
CAP guidelines, there are lots of ridiculous rules, in my humble
opinion. Let the flaming begin!
Jacquie Poteete MT(ASCP)QIHC
Lead Technologist, IHC Lab
Saint F
I too am a research contract lab and would gladly accept donations of expired
antibodies.
Paula Pierce, BS, HT(ASCP)HTL
Excalibur Pathology, Inc.
631 N Broadway Ave.
Moore, OK 73160
405-759-3953
www.excaliburpathology.com
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Histo
What a ridiculous rule.
If the antibody works on a control, THAT is the test for whether it is
useful or not, not an arbitrary date printed on the tube.
Terry
-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of Poteete,
Jacquie A.
Sent: 10 October 2008 15:32
I've done both of those methods. I used the cyro embedder at a mohs lab here
for 2 yrs. It's the next best thing to sliced bread and is great for getting
those epi's down. I will get the vendor info and cat # and post it on the net
today. The last one I bought cost appx $1600.00 but was well wor
Has anyone run into problems with a secondary antibody binding non-specifically
to the tissue?
I am using a mouse monoclonal primary with a standard Vector Elite anti-mouse
secondary, following antigen unmasking, followed by ABC and DAB as usual. The
tissues are human tumors. A couple of them
If it's something we're only going to use less thata dozen times a year, we
sent them to an outside source like US labs, Oncotech, etc. I can't justify
the cost or the tech time to work it up on something rarely used.
~Kim ~
~Don't let your life end before it begins~
OU Rocks
--- On F
You can only use unexpired reagents on patient material. We donate useful
expired (but still working) antibodies to a local histology school. Sending
the reagents to Patsy Reugg is another excellent option.
Patti Loykasek BS, HTL, QIHC
Clinical IHC Supervisor
PhenoPath Laboratories
Seattle, WA
Hello Histonetters,
We've received a request from our breast doc to see if we can find a
sterile ink-inside and out.
It doesn't necessarily have to be India Ink-our PA can re-ink the breast
specimen when it comes to the histo department, but the doc is looking
for something other than the bott
We are not permitted to use anything that is expired, no matter what it
is. Yes, the lab takes a major hit because of this regulation. I send
my expired antibodies to Patsy Ruegg so she can use them for
experimental purposes. It isn't a total loss, just a very expensive one.
Thanks for the remind
We have determined the number of times an antibody is requested/year, and what
the average shelf-life is that a particular vendor supplies. If we determine
that the antibody will not be used by that time, we'll check to see if a
smaller amount can be purchased. If not, we'll discuss the options
We do not use expired antibodies. For those that have a small volume of
requests or are diluted way out there, we buy concentrates in the
smallest volumes we can find. Manufactures like NeoMarkers sell 100ul
sizes. By carefully tracking usage and dilution factors, we rarely have
an issue with ex
CAP question ANP.22432 states: Are all immunohistochemical reagents used within
their indicated expiration dates?
I am wondering what labs across the country are doing to answer this question.
IHC antibodies are very expensive and can be validated on every run that is
made. Some of these conce
What are most labs using for valdiation of IHC's from equipment to
equipment? Do you use your own tissue to make TMA's or do you purchase
them for equipment validation, antibody to antibody? Does anyone have a
good source for ordering TMA's or if you make your own, can you help us
out with your p
Does anyone have a catalogue # for the Pepsin used in Brigati's stable pepsin?
Thanks,
Gene
Cleveland Clinic
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I am a cytotechnologist and for making cell blocks I use 25.occ specimen in
a 50.0cc conical tube add 95% alcohol to bring to 45.0cc. You will notice
a precipitate will form. Mix well. Centrifuge for 10 mins and then let
sit for at least 1 hr. You will find there is a hard button at the bottom
hello all, could someone please share with me the recipe/protocol for
Toluidine blue stain (Sterchi and Eurell)? I want to try a combination with
Macneal"s tetrachrome
Much appreciated. have a great weekend
--
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Af
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