Hi everyone!
Thank you for all the great responses to my last question about metal
vs. plastic molds. I have another question being debated however, How to
dispose of slides once the required time is up (10 years for us). We
have put the slide in sharps containers and then into biohazard, but are
I have a refurbished Reichert-Jung 2030, but with a knife holder from a
newer model (so I'm told by the refurbisher). I use 0-1 degree angle.
Merced
--On Tuesday, March 10, 2009 4:15 PM -0700 Va Paula Sicurello
vapat...@yahoo.com wrote:
Hello Listers,
I have inherited a Reichert-Jung
Hi All,
I desperately need to get a new incubator oven for my histology lab. It
seems as if most ovens are now convection ovens. Since my old oven is
not convection I am just concerned that the constant air movement will
some how affect the tissue slides in the way the paraffin in melted
We have a broken glass waste container we put ours in for pick up and
disposal (don't deal with non-animal patients, so not an issue).
-M
--On Wednesday, March 11, 2009 4:56 AM -0400 Sharon Campbell
shar...@celligent.net wrote:
Hi everyone!
Thank you for all the great responses to my last
Old slides, after all the processing they have endured are not hazardous
(from the contagious/disease point of view) any more.
They are hazardous from the mechanical (potential physical injury point of
view) and it is more than enough to dispose of them in sharps containers.
Word of caution,
Usually all ovens (new and old are of the convection type) and the air
circulation will improve the effect of heat over the slides.
René J.
--- On Wed, 3/11/09, Kalleberg, Kristopher kristopher.kalleb...@unilever.com
wrote:
From: Kalleberg, Kristopher kristopher.kalleb...@unilever.com
Subject:
Hi everyone,
Does anyone have any experience in IF on mouse embryo frozen sections?
Vanessa
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For ease of reference, if you are asking, replying, or quoting anything
pertaining to CAP regs, PLEASE give the CAP checklist number that contains the
information being discussed.
For those of us trying to keep current, it makes it so much easier to check our
own records.
Just a suggestion -
Disclaimer: I do not consider myself to be a coding expert. Take my opinions
with a grain of salt.
I've never seen coding for softening of keratin therefore I don't see how you
could charge for it. This is not decalcification and should not be represented
as such. Until such time as a code
Metal molds vs Disposable molds
We too have chosen metal molds. We clean them by placing them in a
random processing basket and running an extended purge cycle with xylene
and alcohol. It has worked very well for us.
Slide disposal:
I believe the CAP guidelines from 2005 state that slides should
Hi Kris,
I think that a smallish lab oven would suffice. If all you are doing is
melting paraffins I think any of the ones from the major vendors (Fisher, VWR,
etc.) would be good enough.
I do believe that those are still just ovens and don't have the air currents.
Paula Sicurello
VA
We use the same company that takes away our alcohol waste. They pulverize
the slides for us. There is a company in WI that makes a Slydeater that you
can rent for one month to pulverize your slides. I am looking into that
aspect.
Joyce Cline, H.T. (ASCP)
Technical Specialist
Hagerstown Medical
Sean:
Considering that the van't Hoff coefficient determines a metabolic coefficient
increase/reduction every 10ºC (Q10) I would increase the processing
period one time every 10ºc between the temperature your protocol was designed
to work at and the new temperature you are going to use now.
hi everyone,
last week i had aquestion regarding xylene substitutes and Vector Blue
compatibility. i came across an article saying use of paraffin oil as
substitute for xylene...
can paraffin oil be used as clearing agent ?
When our histology lab receives a compromised specimen (not labeled with
patient's name, wrong DOB, no label whatsoever, etc) we have the offending
party correct the error and fill out an accountability form accepting full
responsibility for the identification of the specimen. We then scan the
we treat with SHARPS + Biological Hazardous
2009-03-12
TF
发件人： Mike Pence
发送时间： 2009-03-11 21:29:29
收件人： Sharon Campbell; histonet@lists.utsouthwestern.edu
抄送：
主题： RE: [Histonet] slide disposal
All of our slides are put into buckets and placed in the regular
dumpster as long as
hi, i am trying to stain the rat CD31
the cat: 550274 is the anti-mouse CD31
have anyone tried this one:
http://www.bdbiosciences.com/external_files/pm/doc/tds/rat/live/web_enabled/22711D_555025.pdf
test it on parafin sections?
2009-03-12
TF
发件人： Chiriboga, Luis
发送时间： 2009-03-07
Thank you Joyce -
The SlydEater pulverizes the glass slide and obliterates the label
affixed to the slide. Units are available for both short term and
long term rentals, as well as Least to Own.
If interested, visit our website at:
http://www.SlydEater.com
or by calling me directly at
Hi Histo netters,
I am am my wit's end. I know how to section but my sections are compressing
like crazy. I've altered my processing protocol thinking that I was
overprocessing my samples.
I've tried different knife angles, different brands of razor blade knives.
It's either me or the
Hi Rene,
To clarify your post: Do you mean liquid dish soap (like palmolive) is to be
used? When you say not dishwasher detergent I think of Automatic Dishwasher
detergent such as Cascade.
Or no dish soap at all and I should use liquid laundry detergent? Thanks.
Leslie
From: Rene J Buesa
What animal tissues are you using? If rodent, may need less time on the
steps - like 10-20 min.
--On Wednesday, March 11, 2009 11:29 AM -0700 Va Paula Sicurello
vapat...@yahoo.com wrote:
Hi Histo netters,
I am am my wit's end. I know how to section but my sections are
compressing like
Posting for a friend..
We have hit a bump in the road with our IHC test and thought maybe you
might have some input as we seem to be stuck. We stain (typically for
virus) frozen bovine tissues using Biocare's Mach-3 AP polymer kit and
develop with Vector Red, counterstain, and
Rene,
I agree with your process as to validation, however, could you help me
understand where does the CAP state (what check off list item)
concerning validation requirements when changing out processors or
processing technologies. My staff is currently performing the validation
process with a
Is anyone performing IHC on FFPE human tissue for HTLV-1?
Many Thanks,
Jim
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Yes!! Paraffin oil = mineral oil.
Under separate cover I am sending you an article I wrote on the subject.
René J.
--- On Wed, 3/11/09, anjan kumar drvet_an...@hotmail.com wrote:
From: anjan kumar drvet_an...@hotmail.com
Subject: [Histonet] paraffin oil-clearing agent
To: triple
As long as everything is solved and there is no doubt about whose specimen it
is, I don't see any benefit in letting people know that is was initially
compromised.
René J.
--- On Wed, 3/11/09, Bell, Lynne lynne.b...@cvmc.org wrote:
From: Bell, Lynne lynne.b...@cvmc.org
Subject: [Histonet]
Leslie:
If you use Cascade, Palmolive or any other dishwasher liquid soap the paraffin
will be dissolved and you will ruin your sections.
I am referring to laundry liquid soap or mild hand washing liquid soap.
René J.
--- On Wed, 3/11/09, Leslie Chen lc...@mednet.ucla.edu wrote:
From:
Compressed sections usually are the result of:
1- blunt knife
2- too soft paraffin for the selected thickness
3- too warm paraffin (block not cold enough)
4- blade too vertical (not enough clearing angle)
5- cutting too fast, specially with thin sections
6- too warm blade
7- microtome set screws
Did you change paraffins lately? Softer paraffins, ie., lower melting point,
have more compressibility.
Peggy A. Wenk, HTL(ASCP)SLS
Beaumont Hospital
Royal Oak, MI 48073
- Original Message -
From: Va Paula Sicurello vapat...@yahoo.com
To: HistoNet histonet@lists.utsouthwestern.edu
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