Just be sure it is an open system that will allow you to use any primary
antibody you need and secondary antibody not just a secondary kit the company
provides. You will need to confirm that if you are using a chicken primary for
instance, you can get and use a secondary link that will work wit
I guess I would have to echo that as well!
Cheers!
Greg
Greg Dobbin, R.T.
Chief Technologist, Anatomic Pathology
Dept. of Laboratory Medicine,
Queen Elizabeth Hospital,
P.O. Box 6600
Charlottetown, PEC1A 8T5
Phone: (902) 894-2337
Fax: (902) 894-2385
"I find that the harder I work, the
more l
I echo everything Julie said!
Greg
Greg Dobbin, R.T.
Chief Technologist, Anatomic Pathology
Dept. of Laboratory Medicine,
Queen Elizabeth Hospital,
P.O. Box 6600
Charlottetown, PEC1A 8T5
Phone: (902) 894-2337
Fax: (902) 894-2385
"I find that the harder I work, the
more luck I seem to have."
Hi All,
I have been receiving numerous inquires as to why I have left PhenoPath. I
appreciate your inquires and congratulations. I am going to give a blanket
reply, since I have time constraints.
PhenoPath is a great company and I have the highest regard for Dr's. Allen
Gown, Steve Kussick
Hi Doug and Histonet,
Thanks for the clarification on the company tree. You are absolutely correct!
Have a great weekend fellow histonet subscribers! As for me, I'm packing and
heading back to the SF Bay Area. I have accepted a position as a Histology
Manager for a private reference lab.
The EPA governs the environment. There are two major Federal EPA Regs for
water: Clean Water Act (CWA) of 1972 & amendments in 1972. Second is the
Safe Drinking Water Act (SWDA) with Ammendments dated 1996. The first
governs discharge into navigable waters and surface water contamination
concerns
Hi,
FYI Posting from a TBS recent article:
Quote:
Fisher Scientific Merges with Apogent and then is acquired by Thermo
As you have probably heard, Fisher Scientific, one of TBS' premier dealers,
first merged with Apogent - an international manufacturing company having
several divisions that pro
Hi,
I'm trying to locate some images of crystal fall out when staining with fast
red. Does anyone know where I can look?
Keri Colwell
Laboratory Technologist
CFIA - Lethbridge Laboratory
P.O. Box 640
Lethbridge, AB
T1J 3Z4
Phone/ Téléphone (403) 382-5500 ext. 5613/ Facsimile/Telecopier: (403)
We store our partically used blades in a centrifuge tube. It works great.
Pamela Romundstad
Gundersen Lutheran
1910 South Ave No.
LaCrosse, WI 54601
608-775-3139
histonet-request@
Can't speak highly enough of the BondMax form Leica Microsystems. If
cost is an issue and you're looking at what to avoid, IMHO avoid
anything Ventana
Ronnie Houston
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450
-Original Message-
From: histone
Formaldehyde can mess up the bacteria that treat the sewage. We used to
neutralize our formaldehyde with Richard Allen's "Vytac" and send the results
down the drain. Two years ago we were told that we would have to get a license
and do quantitative analysis on each batch in order to continue u
I suggest using the Leica Bond-Max. I, an HT(ASCP)cm, did not have any IHC
experiance, but the Bond-Max makes it happen. Just putting dried slides on,
patient info in the computer, slides labeled and then in 3-4 hours, it's all
done. Just dehydrate and clear. Very simple and now giving me time
Happy to be corrected~~thanks Ruth! Does anyone have access to the written
EPA regs on this--I need to send them to a few people who, like me, are a
little behind the times!
Cheryl
-Original Message-
From: Yaskovich, Ruth A (NIH/NIDCR) [E]
[mailto:ryaskov...@dir.nidcr.nih.gov]
Sent: Fri
I am in a veterinary diagnostic lab. In the past we have had very few requests
for IHC and have always sent slides out to another lab to perform IHC as
needed. It is time for us to start doing our own and join the modern age. We
have several certified technicians, but none have experience wit
Hi All-
There is no 'one' answer to this. Small reference labs may be under different
regs than a 500 bed hospital even if they are in the same ZIP code.
Talk to your management and gain permission to call the local water and waste
authority. Please go through proper chain of command befor
Hi All-
There is no 'one' answer to this. Small reference labs may be under different
regs than a 500 bed hospital even if they are in the same ZIP code.
Talk to your management and gain permission to call the local water and waste
authority. Please go through proper chain of command before
Hi All-
There is no 'one' answer to this. Small reference labs may be under different
regs than a 500 bed hospital even if they are in the same ZIP code.
Talk to your management and gain permission to call the local water and waste
authority. Please go through proper chain of command before
Me again!
To those of you that do formalin fixation in the microwave, would you please
share your protocol?? I am having issues
Roxanne
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/hist
FYI... This is from Todays Fierce Pharma Report:
Today's Top Stories
FYI... This is from Todays Fierce Pharma Report:
1. Roche to cut 1,500 jobs in "California cowboys" deal
By Tracy Staton
Deal dissection of the day, No. 1: Now that Genentech has finally conceded to
Roche's buyout plans, the po
And here I've been wondering how to keep my used blade overnight so I
can use it the next day for facing... I've been wrapping it in a 4x4
gauze and squirreling it behind my blade dispenser in a cubbyhole in my
BalanceBank. I am the only one in my lab, so having someone come across
it was not an i
So I guess another analogy would be that's OK to dump any chemical into the
ocean even though we know that it kills life. Some countries do this and say
it's OK while the more thoughtful ones have strict rules about it. Think about
it...Formalin kills, can you assure that it will be dissipated e
I've worked at several places and each one is different on treating
formalin.
The best one I like is to use the Formalin recycler from "Creative Wastes".
It's not electric, just works off of gravity and layers of filters. Less
formalin to buy, don't have to buy neutralizers and don't have to dump
FYI... This is from Todays Fierce Pharma Report:
Today's Top Stories
1. Roche to cut jobs in "California cowboys" deal
By Tracy Staton Comment | Forward
Deal dissection of the day, No. 1: Now that Genentech has finally conceded to
Roche's buyout plans, the postgame analysis begins. As we all k
Jennifer,
Ooh, I like that! I'll start doing the same first thing Monday.
Funny how such a simple fix was literally sitting in the drawer next to
the microtome all along!
William (Bill) O'Donnell, HT (ASCP) QIHC
Lead Histologist
Good Samaritan Hospital
10 East 31st Street
Kearney, NE 68847
Hi all,
I'm sending this for the head of our immuno department. She's asking if anyone
has had success with the Cox2 antibody in FFPE human tissue. I'll pass any
replies on to her.
Thanks,
Barbara Albert
UCSF Medical Center
San Francisco
___
Histo
I thought the replies would be more "cutting edge".
What I do is the most low tech of all, I put the still usable knife in a box
(usually the empty box from the slides we use) and then use the blades when
needed. Just be careful to keep the pointy part away from your fingers.
All histotech
someday, people are gonna learn that theres a self-service unsubscribe
link at the end of each and every mail sent from this list ;)
Tina Haas/mhhcc.org wrote:
Tina Haas, HT, ASCP
Section Head, Pathology Department
Memorial Hospital and Health Care Center
Jasper, IN 47546
Ph#812-482-0291
Fax#8
Tina Haas, HT, ASCP
Section Head, Pathology Department
Memorial Hospital and Health Care Center
Jasper, IN 47546
Ph#812-482-0291
Fax#812-482-0447
th...@mhhcc.org
please unsubscribe from histonet. thanks!
"Whitaker, Bonnie"
Sent by: histonet-boun...@lists.utsouthwestern.edu
03/13/2009 01:4
I highly suggest Biocare. It is nice to use a company that still cares about
their customers. I love their products/equipment. They have great customer
service, sales reps. and their service/tech help is top notch. I made the
switch years ago too.
Kelly D. Boyd, BS, HTL (ASCP)
Lab Manager
Har
I agree. It is up to each state/county/city/municipality or whatever to
determine what their regulations are, and facilities must comply with the
most stringent set of rules that they are governed by.
Bonnie Whitaker
Clinical Histology Manager
Ohio State University Medical Center
614.293.5048
You are correct Nanette. Waste water treatment is under the jurisdiction of
local regulations from place to place. What's allowed in one place may not be
in another. Seems it depends on what various waste treatment facilities have
the ability to handle. It also seems, historically what has h
I've worked where it was acceptable by the city sewage authority to dump
formalin down the drain. I've also worked where
it was collected by a company as hazardous waste. Here, we collect and
neutralize before drain disposal, a much more sensible method.
As for dispo blades that are not quite "
I disagree also, it is up to the local wastewater treatment plant to decide if
you may dispose of formalin down the drain, they also dictate how much. It is
not prohibited across the board..
Rosa Fields, HT (ASCP)
Gastroenterology Specialties
Histology Supervisor
4545 R Street
Lincoln, NE 6850
I will save a blade in a plastic 5-slide mailer. I usually use it for
trimming so that I don't waste a new blade. I label the container with
"sharps" info.
Jennifer
"Webb, Dorothy L"
Sent by: histonet-boun...@lists.utsouthwestern.edu
03/13/2009 10:03 AM
To
histonet@lists.utsouthwestern.
I have to disagree. Although it certainly is not environmentally friendly to
dispose of formalin down the drain, it is not prohibited. At the hospital
where I worked, a city inspector came to the lab and and designated in writing
how many gallons of formalin could be put in the sewer system pe
Histoland:
I am having problems sectioning spleen without artifact(holes) as well as
pencil shaves. A little bit of background mouse spleen fresh frozen
embedded in OCT (plastic mold) at -80C freezer. The tissue was then given to
me already frozen in OCT mold. Each specimen consisted of
Ugh. We are not allowed to dispose any chemical, including formaldehyde,
down our drains here at UB (Buffalo, NY). Everything goes into
appropriately-labeled waste bottles for Hazardous Waste to dispose of,
however they do it.
--On Saturday, March 14, 2009 12:05 AM +0800 TF wrote:
Hard to
Happy Friday the 13th Histonet!
Forewarning, this is a little long. There seems to be a lot of discussion as
of late, to all the buy outs, and who owns who. I have been following this
trend for several years with great interest. It is amazing how pricing,
customer support and service, varie
i "store" them in the sharps waste container ;)
Webb, Dorothy L wrote:
Kind of a "Friday" type of question inasmuch as it could open some
"cutting" remarks...how do others handle microtome blades that are
not totally used? Our situation is that we use the more expensive
"teflon coated" blad
Kind of a "Friday" type of question inasmuch as it could open some
"cutting" remarks...how do others handle microtome blades that are
not totally used? Our situation is that we use the more expensive
"teflon coated" blades for certain tissues that are more delicate or
difficult to obtain the m
Jessica:
It is absolutely prohibited, "verboten" to dump formalin into the sewer system.
Try to check out other recommendations by this "consultant" and take them with,
at least, a grain of salt.
That guy does not know what is talking about and could get your lab in serious
trouble.
René J.
---
Hi Margaret,
You are right you can take the girl from Boston but not Boston out of
the girl. Not to be picky...Bostonian...or has it been changed :0
My entire family is there and I go visit every summer.
-Original Message-
From: Sherwood, Margaret [mailto:msherw...@partners.org]
Sent: Fr
Hard to say...
we perfuse sooo many animals everyday...several litres for one lab
into the sea
2009-03-14
TF
发件人： Jessica Piche
发送时间： 2009-03-13 23:25:02
收件人： histonet
抄送：
主题： [Histonet] Disposal of Formaldehyde
?
Hi All,
We have a question regarding the disposal of forma
Here's a message from Jennifer(please respond to the list or her
directly thanks)
Hi everyone,
We are getting ready to start running immunos at our lab and we need to
install a DI water system. After talking to a friend who recently put in a
system at his lab, I realize that there
We collect ours and the Hazardous Waste Dept of the hospital collects
it. Now what they do with it I have no idea. We collect all our waste,
fixatives, stains etc. I know of at least 2 facilities in Houston that
pour theirs down the drain.
I am from Boston I was so pleased to read the steps they
We dump very little down the drain. Formalin has not been drained dump since
1988. We used to have it hauled away, because the former administration never
got into re-cycling the formalin.
What we do today, is too de-formalize the formalin, test it and then drain
dumped if it passes. We buy the
The same recycler is used here for us. we are absolutely not allowed to put
formaldehyde/formalin or EtOH or Xylene down the sink. If you don't use a
recycler, hire a waste company to handle it.
Janet
From: histonet-boun...@lists.utsouthwestern.edu on behal
We are discouraged from putting most any chemical down the drain. The MWRA
(Massachusetts Water Resources Authority) monitors mercury levels, etc. in
Boston Harbor (as well as waterways throughout the state) and will issue steep
fines if labs are found to be dumping such waste down the drain. Con
First ... even if the local authorities allow this ... it doesn't make "Green"
sense to do it, especially when there are other very workable alternatives.
The path we chose was to purchase a very simple gravity feed recycler produced
and sold by Creative Waste Solutions (888) 795-8300. Check th
I'm in Indiana. Here, it is up to the city. We dump all of ours down the drain
and supposedly the city has ok'd that. They say that our hospital is big enough
that all of the water used here will dilute the formalin enough to make it ok.
However I had heard, back when I was in school that formal
Hi All,
We have a question regarding the disposal of formaldehyde. We were told at our
hospital that a consultant said it was okay to dump formaldehyde down the
drain. I believe they said it was okay to dump 15 gallons or so a day! We are
not to fond of this idea and would like to know what e
I would disagree as I am on my third Shandon in a long career and have never
had a problem with any of them. Age and growth in nneds got them. Currently
we use a Pathcentre and it has been excellent. If you haven't used one it is
hard to comment on how they work and how the company works with
I will be out of the office starting 03/13/2009 and will not return until
03/23/2009.
I am on vacation. Since I will be out of the country, I will not be
checking emails. I will respond to your email when I return on March 23,
2009. Thanks!
___
Hi all,
I have a query from a colleague doing research on neuroanatomy as to whether
it is possible ( with relative ease) to cut 20mu sections from JB4 resin
embedded tissue? Apparently these sections ae to be stained and then used
for stereomicroscopy. My experience is not that extensive to be a
I have not heard much good about the Shandon processor. For reliability go with
a VIP every time. This comes from MANY years in the field. I have never had
problems with one.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern
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