[Histonet] ATP 9.4 AND ATP 4.3

Does anybody know tips how to do the muscle biopsy for the above tests? Especially the preparation for the reagents needed. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Re: Fwd: foxp3 ab ihc

http://www.immunoportal.com/modules.php?name=gallery2g2_view=keyalbum.KeywordAlbumg2_keyword=FoxP3 Marilyn Tyler Tue, 17 Nov 2009 23:55:18 -0800 Does anybody in histoland use any FOXP3 antibody. I would like to know which is the better ab to use for FFPE human material. Posting on behalf of

[Histonet] IHC in Ontario Canada

Hello, I've recently started working back in Canada and I am looking to find other techs that are familiar with the OLA regulations for IHCs. Seems to be a little different than the CAP guidelines. Sheila Adey HT MLT

FW: [Histonet] ATP 9.4 AND ATP 4.3

Tay, You'll also need pH4.6 for the acid reversal and fibre typing. In my hands, the technique published by J.M. Round, et al, 1980. Histochem. J. 12 707-710 is the most consistent. All the solutions should be freshly prepared and pH adjusted using a newly calibrated pH meter. The

[Histonet] CD68 on FFPE human tissue

What antibodies are people using for this marker? Kim  Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] CD68 on FFPE human tissue

PG-M1 from dako 2009/11/18 Kim Merriam kmerriam2...@yahoo.com What antibodies are people using for this marker? Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] In search of a reliable androgen receptor

We are trying to find a reliable AR antibody that works on human, primate, rat and mouse, bouins fixed, paraffin embedded tissue. Over the years we have used various antibodies but just recently we have had major problems with inconsistency and between batch variations. For many years we used a

[Histonet] OSHA shoe requirements for histology

It is my understanding that OSHA requires some sort of non-slip shoe to prevent falls due to parafin on floors.  Does anyone have any more information regarding this? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

RE: [Histonet] Re: Freezing of the sceletal muscles

Galina, Get a FLUKE 51-2 electronic thermometer, then get the thermocouple that detects below -100C. Thermocouple Temperature Measurement accuracy: Above -100 °C (-148 °F) J, K, T, E, and N-type** ±[0.05% + 0.3°C (0.5°F) ] R** and S-type** ±[0.05% + 0.4°C (0.7°F) ] Below -100 °C (-148 °F):

Re: [Histonet] OSHA shoe requirements for histology

That one and also not using open shoes. René J. --- On Wed, 11/18/09, richard wells wa7b...@yahoo.com wrote: From: richard wells wa7b...@yahoo.com Subject: [Histonet] OSHA shoe requirements for histology To: histonet histonet@lists.utsouthwestern.edu Date: Wednesday, November 18, 2009, 11:36 AM

[Histonet] IHC in Ontario Canada

Hi Sheila, What are the Ontario Laboratory Accreditation requirements questions that you have regarding IHC? We were assessed in spring/09. We run well over a hundred markers using three Ventana BenchMark XT's. Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston,

[Histonet] Rat perfusion protocol- what's wrong?

Hi all, We are trying to harvest muscle and nerve tissue from Sprague-Dawley rats (upper leg muscle, sciatic nerve). Is it necessary to perfuse the animal before harvesting muscle / nerve, or will simply soaking in 4% PFA do? Our perfusions often, but not always, fail. By fail, I mean the rat does

[Histonet] RE: Histonet Digest, Vol 72, Issue 19

Hi Shiela, I went through an OLA accreditation before I came to work here the States and it seems to me if you do all the same QC you did for CAP you will be OK. You will need to validate your instruments and all of your IHC protocols and have records to show. All of your procedures for all

RE: [Histonet] Fwd: foxp3 ab ihc

Marilyn, We have used Biolegend's Rabbit anti FoxP3 (Cat No 623801) at a 1/20 dilution with citrate retrieval (ER1) on the Bond Max with very good results. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318

[Histonet] Sanderson's Rapid Bone Stain and what you can do about it

It was good hear that RBS was being picked up and sold by Dorn and Hart, but you can also make this up yourself in the laboratory. Cathy Sanderson/Mayton found a way to make this up easier than the original formula and Surgipath manufactured it. The original name was Stevenels Blue and the

[Histonet] RE: foxp3 ab ihc

Marilyn,Clone 236A/E7 (Abcam) worked fine for us. See: De Boer et al. (2007) PlosOne 8:e779 and De Boer et al. (2007) JHC 55:891-898.Cheers, ChrisChris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The NetherlandsDate: Wed, 18 Nov 2009