[Histonet] Looking for the Dako rep for south western Ontario

I'm looking for the Dako rep in South western Ontario, Cda. Thanks. Sheila Adey _ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

RE: [Histonet] Slide writer

In our new Derm Path lab we went with Leica for slide labeling and block labeling, we designed our own computer program with bar coding and other identifiers and the labelers plugged right into our system, they are not cheap but we really like them. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635

[Histonet] CISH - hybridizer

If you do not have heat on your IHC stainer, what instrument do you use for denaturing and hybridization? Does anyone have experience using the MJ Research PTC -100 with in situ slide block from Pegasus Scentific? Thanks, Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI

[Histonet] RE: CISH - hybridizer

Dako has a Hybridizer that holds 20 slides. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu

[Histonet] anti rat mast cell protease

hello. does anybody has experience using santacruz anti-rat mast cell protease [immunofluorescence] in paraffin embedded tissues? i´m aware that it works fine with frozen tissues but don´t know if it works on paraffin. thanks in advance. felipe beraldo laboratory of nephrology state

[Histonet] NSH One Day VIR Forum Opens Registration

Registration is now open for the National Society for Histotechnology One Day Forum in Veterinary, Industry Research Histology. The Event is scheduled for Saturday, March 27, 2010 in Bethesda, MD, 8:15 am – 4:45 pm. Registration Fees Member: $119Non Member: $159 Sessions Include:

[Histonet] Biopsy Processing Schedule

Hello Histonet, I was wondering if anyone can share their Biopsy Processing Schedule with me? We are a research lab and for the first time we will be processing biopsy specimens. We are concerned that our current processing schedule will dry out the tissue too much. Your help is greatly

RE: [Histonet] Biopsy Processing Schedule

You will need to tell us what type of a tissue processor you will be using? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Wahlberg, Nikki Sent: Thursday, January 21, 2010 9:04 AM To:

FW: [Histonet] Biopsy Processing Schedule

Oh Sorry, We have a Leica ASP300. Nikki -Original Message- From: Mike Pence [mailto:mpe...@grhs.net] Sent: Thursday, January 21, 2010 9:10 AM To: Wahlberg, Nikki; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Biopsy Processing Schedule You will need to tell us what type

[Histonet] Over baked slides

Our drying oven misfunctioned over the weekend, heating up to 200 degrees. Needless to say, our tissue in the slides that were in the oven got fried. Does anyone know or has a recipe for us to rescue some of these slides? ___ Histonet mailing list

[Histonet] Leica rep

Hello, does anyone know who the Leica rep in the midwest states Iowa area ? Marcia Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 641-422-7907 ___

[Histonet] Tumor slides

Hello, How are folks working there tumor case slides. We have Tumor review each week and we are working with 5 pathologist that all have different styles of work around for reading cases. One of our time issues are looking and pulling slides for review. Slides

RE: [Histonet] Leica rep

Well, I can tell you this. The reps do monitor this site. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Marcia Funk Sent: Thursday, January 21, 2010 11:00 AM To: histonet@lists.utsouthwestern.edu Subject:

RE: [Histonet] Tumor slides

Our pathology resident takes care of this for us, along with our transcriptionists who pull slides. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's WaySlot 820 Little Rock, AR 72202 phone 501.364.4240 fax501.364.3155 visit

[Histonet] Movats on mouse aortas

Hi, I have had a problem with splotchy elastin staining on certain sections of mouse aorta. I use the Modified Movats and do not use a microwave. But from this one lab some areas of the elastic fibers will retain the hematolylin after differentiation while some areas lose it completely, all in

[Histonet] tissue retention times

OK, everybody crack open your JCAHO requirement manuals... We are a MOHS lab and were surveyed by Joint Commission about 2 months ago. During the survey, we were cited because we only retain our physical tissue specimens for 24 hours, as they remain fresh and are not fixed. We throw the tissue

[Histonet] Reliable Bio repair/ refurbish contact in the Omaha area

I am in need of a Reliable Bio repair/ refurbish contact in the Omaha area. I am less than satisfied with my current one, I have a 15 year history with them, but sadly I question their quality and integrity. The poor service is so blatant it is now become a reflection of me in my superior's

Re: [Histonet] Over baked slides

They are dead as an over fried fish or a burnt cookie. Cut them again, they will be essentially worthless, no matter what you do. René J. --- On Thu, 1/21/10, oscar gonzalez ogv_hist...@yahoo.com wrote: From: oscar gonzalez ogv_hist...@yahoo.com Subject: [Histonet] Over baked slides To:

RE: [Histonet] tissue retention times

We are a small Mohs operation, but we just process and paraffin block all our remaining specimens and file them with the rest of our pathology cases. This worked for CAP anyway. Kim Kolman, HT, (ASCP) VA Eastern Kansas Health Care System Leavenworth, Ks 66048 -Original Message- From:

[Histonet] Laura Miller is Out of the Office.

I will be out of the office starting 01/21/2010 and will not return until 01/22/2010. I am on vacation today. I will respond to you tomorrow when I return. __ This email has been scanned by the MessageLabs Email Security

[Histonet] Microwave Tissue Processing

We are getting a microwave tissue processor and the question has come up as to whether or not a lab aide is allowed, per Florida requirements, to run the processors. These processors are semi-automatic and require involvement by staff to go from one step to the next. Does anyone have any

RE: [Histonet] tissue retention times

We do the same. We aren't a MOHs operation but we do many frozen cases and we process the tissue, cut a permanent processed slide of the frozen sections and then retain them for 10 years along with our other blocks. Cheri Cheryl Miller HT ASCP CM Histology Supervisor Physicians Laboratory

[Histonet] Tendon processing question

We are looking for the best way to process tendon (specifically rabbit) that has been severed and then repaired. Currently they are removing the tendon, rinsing it in PBS and then putting it in a cassette in formalin. The problem I am having is that there appears to be some calcification or

[Histonet] biopsy cassettes

For tiny or friable fragments of skin or keratinaceous aggregate debris or mucoid specimens, one method is to use pieces of hair curler wraps to place the tissue in and then place the specimen in regular cassette or in between sponges in cassette. You wont lose your specimen and it will process

[Histonet] Chemical outdates

Hello Histonetters, We just got inspected by CAP and it was recommended that we put expiration dates on our chemicals once we open them. What kind of time frame do any of you other labs do in regards to this? Thanks for your input, Amylin Johnson

[Histonet] RE: Reliable Bio repair/ refurbish contact in the Omaha area

Have you tried Tom Buck with Hawkeye biomedical -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Thursday, January 21, 2010 12:05 PM To: histonet Subject: [Histonet] Reliable Bio repair/

RE: [Histonet] Slide writer

It writes directly on the slide. Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pru...@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of

[Histonet] To detect injected mouse antibody inside mouse tissue

Hello,   I have a question regarding detecting injected mouse antibody in mouse tissue by IHC. Now I have mouse liver paraffin section. This mouse was injected mouse antibody. I was asked to locate this antibody. This is a mouse chimeric antibody. Variable regions from human antibody was

[Histonet] Glyoxol vs Formalin

What changes should one expect to do when changing from a glyoxol fixative to formalin? Client has been successfully processing prostate biopsies on a Milestone RHS for several years. They are now looking to switch to formalin in anticipation of the need for future molecular studies.

Re: [Histonet] To detect injected mouse antibody inside mouse tissue

Amy, In my opinion the answer is, Yes, certainly possible.  Also in my opinion the answer is No, probably impossible.  Even with a Yahoo address I'm assuming that this is a research/biotech model and project.  In a previous life, when quietly gagged, I went about this on numerable

[Histonet] tamponated formaldehyde

Hi; Can someone please explain to me what is meant by tamponated formaldehyde ? Thanks Sue Sue Reilly Histechnologist. Histology Unit. School of Marine Tropical Biology. James Cook University,Townsville. QLD. 4811. AUSTRALIA P (07) 4781 4181 F (07) 4725 1570 E sue.rei...@jcu.edu.au

RE: [Histonet] tamponated formaldehyde

They mean buffered. It's a poor translation from Italian/Spanish/portugese (BTW, found this answer on Histonet!!) Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu