RE: [Histonet] Dako Rep

Here ya go caren.mie...@dako.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Drew Meyer Sent: Tuesday, February 09, 2010 13:22 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Dako Rep Does

[Histonet] light staining

Hello Histonetters We are having trouble with light staining from the hematoxylin. It is the same lot number we have been in. We changed it out and still the same. All reagent containers were emptied, cleaned and refilled yesterday. We have not changed anything with the timings on our

Re: [Histonet] light staining

Do you use city water in your wash stations? Have you had more snow/ice than usual? If so the Water Tx Plant is putting more chlorine in the water than usual. This will affect you HE. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park

Re: [Histonet] light staining

Check pH of your washing water. pH too low will result in not staining at all. I'd clean the stainer as well. Hello Histonetters We are having trouble with light staining from the hematoxylin. It is the same lot number we have been in. We changed it out and still the same. All reagent

[Histonet] Liver tissue

I am having trouble cutting FFPE liver sections. I have embedded the tissue manually going from fomalin to water to increasing ethanol to xylene and to paraffin. I can get good sections by soaking the block with ice cold water before taking a few sections but then have to re-soak in order to

[Histonet] Progressive counseling

Hello to all in histoland. I had to come out of the box for this question. What types of criteria do you have for verbal and written counseling, as pertaining to histology functions. Any help in this will be helpful. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas

Re: [Histonet] Progressive counseling

The criteria is simple = whenever what is described in the Competencies or Standards of Performance is not met by the histotech. 1 verbal → 2nd verbal → written counseling. René J. --- On Tue, 2/9/10, Scott, Allison D allison_sc...@hchd.tmc.edu wrote: From: Scott, Allison D

[Histonet] Gill sectioning tips?

Hi everyone, We have been trying to section gills lately and have come across quite a few problems. Does anybody have any helpful hints for fixing/sectioning gills? We are going to try to separate each individual arch but the fish we use are very small so we don't know how that will go.

Re: [Histonet] Gill sectioning tips?

If with small you refer to juvenile is different to an small adult. Both will have supporting material for the gill arches but in juveniles they should be easy to section after a good infiltration and using an adequate paraffin wax to meet the tissue resistance. If you are talking about a small

[Histonet] Crystal indentification

Question, when doing a smear for crystal indentification (Gout) are you retaining the slide and for how long. How are you preserving it? Thank raj ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

RE: [Histonet] light staining

I would have a look at the haematoxylin stained section before you differentiate. Does it look OK? If it does then do not differentiate, blue only. (also someone has not accidently put acid in the blueing solution? Check with apH papers). You might try extending the Hx time. If it still looks

[Histonet] Mohs FYI

Hey gang: Here with some good news for once. I found out today that we WON our appeal to JCAHO over our tissue retaining time. They are giving Mohs labs an OK not to have to retain our tissue!! I guess it is 'processed' tissue instead of 'gross' tissue. Retaining it doesn't does not help