Hi Deanne,
My understanding is that you must get p16 from MTM Laboratories. They
have some sort of monopoly right now. I still have some leftover from
Cell Marque and when I run low or the antibody is about to expire I must
also go to MTM.
I currently use the Cell Marque p16 Ready To Use by
We purchase our p16 through MTM laboratories reference # 9517.
Cindy Pyse, CLT, HT (ASCP)
Histology Supervisor
X-Cell Laboratories
e-mail cp...@x-celllab.com
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf
I've been using the Masson Trichrome kit from Richard Allan since 2001 and I've
always gotten really great results. It is a little more expensive than using
home made reagents but the results are very consistent.
Kelley
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I am working on the validation of our microwave processors and have noticed
that the tissues are a bit dry. Would anyone be willing to share their
protocol timings for their fixation, ethanol, isopropyl and wax impregnation
steps?
We are using the Histos-5 microwaves from Milestone and are
Happy Histology Professionals Day!!
When I was going to school at Marshfield one of the Pathologists told me
that without histotechs his work would be much harder.
Thank you Dr. Krawicz for appreciating the job we do!!!
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If it weren't for a med-tech-turned-pathologist that took me up on my
interest in becoming trained in histology (1967), I would not have just
marked my 41st year as a histologist. Back then, you just had to have
OJT and study independently for your written and practical tests. I had
no idea what
I too stumbled into the profession in 1967 and share Sara's sentiments.
I sent the following to our senior staff as well as the supervisory group of
our laboratory:
Today is the first annual National Histotechnology day and is being celebrated
by the histology staff by a morning breakfast (
Allied Search Partners wants to wish you a Happy Histology Professionals
Day!
Histology professionals are the unsung heroes behind the laboratory doors.
The patient may not know you but what you all do is the absolute best for
them because what you do affects their lives and their loved ones
I have been using IDLabs porcine Y chromosome green label probe on frozen
pig tissue with good results most of the time. You say you denature the
probe and then hold it at 37oC...I would think that would allow the probe
to reanneal to itself since you also hybridize later on at that temp...try
Okay--so I don't proof read very well--that was supposed to be
'histologist'--not hostologist in my last post--although there are days when
hostility abounds in clinical pathology!
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Perhaps what each of us ought to do is write a quick line naming the one
person that got you involved in histology and how they did that. I'd be
willing to gather them and pass them on to NSH so they could post it at
the Seattle meeting. If you'd like to do this, send the email to
At least it wasn't Hystologist!! @:)
Janet
From: histonet-boun...@lists.utsouthwestern.edu on behalf of Marsh, Nannette
Sent: Wed 3/10/2010 10:18 AM
To: 'Amy Johnson'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: HistoDay
Okay--so I don't
Somewhere back in the late 60's and somewhere in Michigan there was a cytotech
named Beryl and a histotech named Charlie Brown that introduced me to the
world of the lab while I was in high school. I fell in love with histology
then and have been ever since then!
So THANK YOU Beryl and Charlie
I can beat that one. My secretary ordered me flowers for today and explicitly
spelled the message for the florist. The card got to me and I am a
histoteshnologist. No one knows us. :)
Shirley
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
I was working at a grocery store deli with a BS in biology when a teacher from
the local technical college started there for something to do over the summer.
He told me about a program called Histotechnology that boasted 100% placement
after graduation. That was enough for me I was interning
CBG Biotech offers hydrometers.
Regards,
Beth Austin
CBG Biotech
1-800-941-9484 ext 200
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
histonet-requ...@lists.utsouthwestern.edu
Sent: None
To:
I got into Histology while I was in the Navy. I was out of MLT school by about
three maybe four months, when my wife who was also active duty finally got
transferred to the same base as I. There were two openings for her, Blood Bank
and Histology. The Navy did not want to put a Med Tech in
Actually, I also thought about how we hold the tissue blocks 'hostage' until we
work our wonders on them :-)
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dianne Holmes
Sent: Wednesday, March 10, 2010
Well--there is some hysteria every once in awhile too
From: Bonner, Janet [mailto:janet.bon...@flhosp.org]
Sent: Wednesday, March 10, 2010 9:53 AM
To: Marsh, Nannette; Amy Johnson; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: HistoDay
At
Just a quick note. I too served with Tom, and worked as an MLT in
hematology, PM shift. Every test was STAT (and so none were STAT, these
were the days most everything was done with minimal automation. We did
about 150 CBC's and diffs plus UA, platelets and coags with a staff of
2. And I think
In 1962 I had completed my first year at Georgia and needed to supplement my
tuition since my parents could not foot the whole bill for my next year. I got
a job at a furniture store as a bookkeeper/secretary. It took me a week to
figure out it was not my future profession. The friend I was
I was in college in Tennessee and my advisor wanted me to make a
decision on a career path. I was interested in science but not in being
a nurse or a physician. She suggested I look into laboratory sciences:
histology, medical technology and cytology. I visited a hospital and
toured the labs.
Hi Histonetters!!
I wanted to take a moment and say Happy Histotechnology Professionals
Day. I really have enjoyed reading about how some of you have gotten
into the field. I have to say that I had only heard of the field when I
started recruiting in histology 8 years ago. When I went to
Thanks to the Air Force and AFIP!
I entered basic training and did not have a guaranteed job. I listed Histology
as the 4th choice in my order of preferences for job placement, having no idea
what in the heck histology was. No one there knew what it was either and I
thought I'd be doing
Does anyone have a competency check off for histology techs that they
would be willing to share. Thanks in advance
Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital
5656 Kelley
Houston, Texas 77026
CONFIDENTIALITY NOTICE:
If you have received this e-mail in error, please immediately
I will be out of the office starting 03/10/2010 and will not return until
03/11/2010.
10.In my absence please ask for Mary Campbell . If this is urgent you can
contact me on my cell phone number 858-472-4266.
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Does anyone know a good repair guy/company for our Microm 550 cryostat in the
San Francisco Bay Area?
Thank you,
PJ
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Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From: Hannen, Valerie
Sent: Wednesday, March 10, 2010 12:26 PM
To: histonet-requ...@lists.utsouthwestern.edu
Subject: Happy Histotechnology Day!!
I had graduated from an MLT program in West Virginia in 1983. Moved to
Florida in June of 1984. I made my
Hey Histonet,
Is anyone using the IL-17A antibody? where did you buy it? where you buy the
secondary as well? protocol?
Any suggestions will be very much appreciated
Thank you
Chakib
Histology/necropsy Supervisor HTL(ASCP)
MIT-DCM
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Histonet
I am really wanting to end the use of Xylene in our lab and considering the
use of SubX from Surgipath/leica. I am currently using Gill III from
Surgipath as well. I was wondering if anyone out there is also using SubX
and if you would share your staining and processing regimen with me and any
Prometheus Healthcare, New York's # 1 Recruiter for Laboratory
professionals, is currently seeking technicians and technologists for
numerous histology opportunities throughout New York City and the
surrounding areas. Shifts vary from day, evening to night and opportunities
are available within
Happy Histotechnology Day to everyone out there in Histoland, whether that
be clinical, research, veterinary, industry, or all of the above. Since we
are sharing how we all got started in Histotechnology, I remember having a
Premed/Biochemistry degree in 1996,(what do you do with that?) shortly
Good Afternoon Everyone,
Vinnie, Chad McMahan and I have just returned from the SC State capital for
Governor Sanford to sign a proclamation for Histotechnology Professional's Day.
It was a rewarding experience having our Governor acknowledge our profession
with the signing of the proclamation.
Way to go South Carolina. Congratulations. Hope your day was as good as mine.
Low key but hey, I am the only one here. Got flowers from my Pathologists.
We can start planning for next year.
Shirley
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
For which tasks?
René J.
--- On Wed, 3/10/10, Scott, Allison D allison_sc...@hchd.tmc.edu wrote:
From: Scott, Allison D allison_sc...@hchd.tmc.edu
Subject: [Histonet] Competency Checkoff
To: histonet@lists.utsouthwestern.edu
Date: Wednesday, March 10, 2010, 12:15 PM
Does anyone have a
Veronica I am pretty sure it was University of Penna. , anyone out there
know different?
Dana
In a message dated 3/10/2010 6:18:02 P.M. Eastern Standard Time,
veronica...@hotmail.com writes:
Happy Histotechnology Professionals Day!
One of my Professors told me that Sheehan(of Theory
Hi Histonet,
I am performing the trichrome stain on cardiac tissue. The procedure calls
for Weigert's iron hematoxylin stain or equivalent. Would Harris
hematoxylin work as well. It's what I have readily available at the lab.
The instructions for preparing the Weigert stain involves mixing fresh
Colorados Governor Bill Ritter also signed a Proclamation
Sent via BlackBerry from T-Mobile
-Original Message-
From: Shirley A. Powell powell...@mercer.edu
Date: Wed, 10 Mar 2010 14:58:36
To: wanda.sm...@hcahealthcare.comwanda.sm...@hcahealthcare.com;
Colin,
Rather than Weigert's Hx you can use the Celestin Blue Harris Hx
sequence.
Stain 5minutes in Celestine Blue (0.5% Celestine Blue (CI 51050) in 5%
iron alum (ferric ammonium sulphate)), followed by Harris Hx 5 minutes,
contine Masson Staining. See:
Henwood T, Llewellyn B, Montgomery I,
No. The PTA or PMA used in Masson's method is too acidic and would remove
Harris's or any other haemalum. An iron-haematoxylin such as Weigert's or
Lillie's gives an acid-resistant nuclear stain that is progressive and almost
black.
John Kiernan
Anatomy, UWO
London, Canada
= = =
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