[Histonet] Apoptosis staining on mouse testis

Hello all, I am trying to stain for apoptosis on bouins fixed, paraffin embedded mouse testis. I have tried a cleaved caspase-3 anitbody, TUNEL, with no success. Any suggestions would be much appreciated. Many thanks Lindsey ___ Histonet mailing

[Histonet] Lyve - 1 on Bond Max

** Proprietary ** ** Reply Requested When Convenient ** Hi there, I was wondering if anyone on the histonet list use anti-mLYVE-1 antibody (purified in rat Monoclonal IgG 2A from RD System) on the Bond Max. We have been trying out various titrations and pre-treatment without much success. All

Re: [Histonet] human on human IHC

Along those same lines I have conjugated primaries to biotin and it works beautifully for human on human IHC. Another alternative if extra amplification is needed is to conjugating to FITC and then using a rabbit-anti-FITC followed by an anti-rabbit polymer. Just be aware with human on human

RE: [Histonet] human on human IHC

Anything using FITC is pretty expensive. If you aren't want= ing flourescence then why would you use this? Does this specific conj ugation eliminate the Fc binding? Conjugating to HRP doesn't seem to = yield that Fc region staining that I can see? Just a happy Monday

[Histonet] vanGieson-Trichrome staining protocol

Is there anyone out there that has done these two stains together? If so, I need assistance with a protocol. Also I am doing a reticulum stain and I am having a time with the ammoniacal silver solution, I can get the solution clear but when it comes to the cloudy do they mean milky cloudy?

Re: [Histonet] vanGieson-Trichrome staining protocol

Adele, Since the vanGieson IS a trichrome stain, why would anyone wish to combine one trichrome with another? Bryan - Original Message - From: Doughty, Adele adoug...@lrri.org To: histonet@lists.utsouthwestern.edu Sent: Monday, July 19, 2010 10:29 AM Subject: [Histonet]

Re: [Histonet] Wax removal

Hi, Thanks for the reminder. We purchased them from Lab Safety Supply. They are called Safety Treads, and they come in a variety of sizes and shapes. The one we have is 6 X 24 in (I guesstimated the size wrong earlier) and it's part number is 10766BR. Here is a link to the web page for them:

Re: [Histonet] vanGieson-Trichrome staining protocol

Are you perhaps refering to the Verhoff's Van Gieson, if you are trying to stain elastic then  a trichrome it can be done.  The silver should be cloudy like a slightly dirty window , not milky  generally if you add enough ammonia to the silver and sodium hydroxide mixture to render it

RE: [Histonet] vanGieson-Trichrome staining protocol

And it looks so pretty (VVG/TRI)- especially on lung. Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

[Histonet] Toluidine Blue and staining archaeological residues.

Hello Histonetters, I am working with archaeological residues lifted from Holocene grindstones. I was trying to find a stain that could differentiate between plant and animal tissue in one hit. I have been using buffered Toluidine Blue solutions however given that these are ancient residues