[Histonet] FX111a Protocol

Does anyone have a current protocol for Factor X111a on the Ventana Ultra that is working for you? I would appreciate any info or support you could give. We are using the AC-1A1 clone from Cell Marque for derms. Thanks! ___ Histonet mailing

[Histonet] Sunquest CoPath Rep

Could any Sunquest CoPath sales rep please contact me privately off the list? Thanks, Drew Meyer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] used Leica XL

I've been asked to get pricing for possible purchase of a good used/refurbished Leica autostainer XL. Gale Limron CT, HT (ASCP) Histology Supervisor This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or

[Histonet] Leica autostainer XL

Does anyone know if you can just deparaffinize slides in the stainer. Im just wondering if I also need a small stain line to dewax specials. Thank you, Nicole ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

Re: [Histonet] Leica autostainer XL

You can set up a just depar run. Sent from myTouch 4G - Reply message - From: Nicole Tatum nic...@dlcjax.com To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica autostainer XL Date: Wed, Jan 26, 2011 9:48 am Does anyone know if you can

RE: [Histonet] FX111a Protocol

Hi, We run FactorX111a clone AC-1A1 on the Ultra, here is our protocol: 1. De-paraffinazation (selected) 2. Cell conditioning #1 20 minutes, then 36 minutes. 3. Antibody for 24 minutes 4. Ultra wash (selected) 5. Hematoxylin 4 minutes 6. Bluing 4 minutes. Hope this helps. Lisa V. Children's

[Histonet] Oil Red O for FS on Muscle

Happy hump day! Does anyone have a good procedure for Oil Red O for FS on Muscle. The procedure the lab I am working with is having a great deal of problems with their muscle biopsy panel. I am trouble-shooting some of the issues for the 1-Step Trichrome 3.4 pH, NADH, AT Pase, I am

RE: [Histonet] Oil Red O for FS on Muscle

Hi, We use Oil Red O - but we don't do it on muscle. Our protocol is we filter the whole bottle before we use it and then we apply it to unfixed slides for about 30 minutes. Rinse in tap water, counterstain with Hematoxylin and coverslip with Aquamount. We use it for cytospins and occasionally

RE: [Histonet] Oil Red O for FS on Muscle

Allison We make up our Oil Red O from scratch same day we use it, let it stand 10 minutes, and then filter with a Millipore Stericup 0.22µm, GP Express Plus Membrane, 250ml Receiver Bottle, catalog number: SCGPU02RE under vacuum. That seems to help a bit with the background and we are working

Re: [Histonet] Leica autostainer XL

I have a run set up to bake the slides run them down to water ( for my specials), another just to start at hematoxylin down to xylene etc. The stainer allows for nearly any custom program you can think of. :o) If you have any questions about how to set the program up, please let me know I

Re: [Histonet] Oil Red O for FS on Muscle

I've done many of these stains and I get my ORO stain from Poly Scientific. Actually I use the whole kit and I use Freida's protocol in the second edition. That includes fixing in 37% formaldehyde. I filter the ORO just before use and usually get nice clean slides. If there is a residue it

[Histonet] Histotech Needed in Santa Rosa, CA.

Certified Histotech needed in Santa Rosa, CA. Part-time (25-30 hrs/wk) opportunity for an experienced and certified tech to work in a brand new GI path lab. Competitive pay and wonderful working environment. Send resumes to tja...@yahoo.com.   Kind Regards,   Timothy Jay Pillar Consulting, LLC

[Histonet] glass cleaner

Hello I am looking for suggestions on the best chemical cleaner for glassware that is used for special stains. Any ideas? Thanks, Nacaela Johnson Histology Technician KCCC Pathology 12000 110th St., Ste. 400 Overland Park, KS 66210 Office: 913-234-0576 Fax: 913-433-7639 Email:

[Histonet] (no subject)

Hello I am really new to the field of histlogy. I wanted to get some opinions on this field vs. cytology. I am wondering which field is better for me. Any suggestions or thoughts are welcome. Thanks!!! I remain yours truely, Candice Camille

Re: [Histonet] glass cleaner

~ 90 mL 70% EtOH ~ 10 mL 25% HCl Removed everything I ever had to deal with. After that give your glassware a good wash in the dishwasher, then rinse with plenty of demineralized water. And wear protective gloves, but as we all do, I actually don't have to remind any of the list members, right?

RE: [Histonet] (no subject)

Go for HISTOLOGY!! Cytology requires a lot of Sitting in One Place Staring into a Microscope (please! - no offense to cytotechs - really!). With histology, your daily routine is varied and invigorating. And now I'm probably on some Cytology Tech Blacklist... sigh...

Re: [Histonet] (no subject)

Is there a chance to go and see a lab which does histology? Hello I am really new to the field of histlogy. I wanted to get some opinions on this field vs. cytology. I am wondering which field is better for me. Any suggestions or thoughts are welcome. Thanks!!! I remain yours

Re: [Histonet] (no subject)

Yes, I just started working in a histology lab. Thats y I was asking this question. I heard about cytology and wanted to get some opinions as to who offered what! Just trying to keep the options open. I doubt you are on any blacklist. LOL!! I remain yours truely, Candice Camille

Re: [Histonet] glass cleaner

HISTOLOGY UNIVERSAL WASHING INSTRUCTIONS FOR GLASSWARE Alternative to Acid Cleaning Glassware 5% Clorox is a very inexpensive and readily available chemical treatment solution, which is an alternative to acid cleaning. All glassware used for special stains MUST be cleaned in the

RE: [Histonet] (no subject)

Of course as most of us would say...histology rocks!! Most histotechs also do cytology is most hospital settings. The only difference is that cytotechs actually have to screen the slides that the histo people prepare. I think that cytologists make slightly more money...but histology is way more

RE: [Histonet] (no subject)

Back in the day when I was trying to decide which school to pick for my scholarship monies, I had a choice of the Med Tech program, the Cytology program or the Histotechnology program. I had absolutely no idea what Histology was but that is the program I choose. One of the best decisions I ever

Re: [Histonet] (no subject)

I just sort of fell into Histology some 40 plus years ago and never looked back. I have left the field and always come back to it. I have done research and clinical and still love it. Pam Marcum UAMS Sent from my Verizon Wireless BlackBerry -Original Message- From:

[Histonet] TGFβ RII

Hi histonetters, I need suggestion and help with the TGFβ RII (D-2): sc-17799 Ab, which should show a cytoplasmic staining. My problem is that I am getting a beautiful nuclear staining only. How I can fix this problem? What to pay attention on and what to change in the usual protocol? My AR is

[Histonet] dual fluorescent staining

Hello everyone, I am working on a protocol for a dual fluorescent stain with two mouse antibodies (one is IgG1 and the other is IgG2a). We started by performing the two stains sequentially with a serum free protein blocking step in between, tagging one antibody with a 488 FITC labeled