No problem Amos, I have a one page handout that I use for workshops and
presentations on how to do the math on this, it can be tricky, so if
anyone is interested just e-mail me. Since we are talking about matched
isotype negative controls, essentially three things need to be taken
into considerati
Thanks Liz,
You are absolutly right there, but have you ever noticed some folks
eyes glaze over when you say that they must both be run at the same ug/mL? I
would be lying if I said it never happened to me until I forced myself to
work it out. Once you do though it isn't too bad. Unfortunately
Hi,
I seldom do this, but I'll bet I know EXACTLY what the problem is. Try
it again and stop the rehydration at 95% ETOH then put it directly into the
Alizarin Red (double check the pH of course). This will surely fix it. The
calcium gets rinsed out when it is rehydrated. The same thing happen
Hi,
There is not any practical way to remove the DAB. I think I read about
boiling it in a salt (of some sort) solution removing the DAB. Honestly I
never bothered trying it as you need to ask yourself "What is this doing to
my target antigen?". You should consider just restaining it with the D
Amos
Isotype negative controls are based upon protein concentration not
dilution. They must be the same protein concentration of the primary
antibody at the dilution you are using. Using the same dilution will
not work unless both stock solutions (primary antibody and isotype
control) are at the
Hi,
This is simply a question of definitions. They are actually both right.
If you have the patient tissue as a negative control you are not using the
primary antibody on it but are replacing it (ideally) with an Ig with the
same isotype AND dilution (universal negative is stupid). So if your
Hi,
BrdU from Sigma cat # B2531 works really well. My primary application
is in mice, however the target for this antibody is the BrdU that was
incorporated into the DNA strand not the animal itself so you should be able
to use it in ANY animal. (Don't forget to denature).
Good Luck,
Amos
M
We are using Biocare Smooth Muscle Myosin-Heavy Chains (AP Protocol
using Warp Red and previously Vulcan Fast Red)on the Nemesis auto
stainer.. Lately we have notice considerable background staining. Any
suggestions. The slides almost look like in the old days when you put
too much albumin ( as an
"If an inspector saw that, you would be on immediate suspension."
This was the last statement from the original post, I chose not to include
it initially but it's just too juicy to keep from everyone else. Having
forwarded several of the responses I think he has come to understand that
it's no
Curt,
Thank you. I'm here all week, try the veal.
Sincerely,
Jay A. Lundgren, M.S., HTL (ASCP)
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Great opportunity for a Histotechnician in a brand new laboratory! We
are a 5 physician gastroenterology practice located in Las Vegas, NV
looking for a certified HT or HTL. Candidate must meet CLIA grossing
requirements. The candidate will be responsible for routine histology
duties. This is a
Hale, Meredith would like to recall the message, "Las Vegas HT Position".
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Great opportunity for a Histotechnician in a brand new laboratory! We
are a 5 physician gastroenterology practice located in Las Vegas, NV
looking for a certified HT or HTL. Candidate must meet CLIA grossing
requirements. The candidate will be responsible for routine histology
duties. This is a
Gayle,
I would be interested in the articles as well. I had a similar experience:
nerve samples from rats were being shipped to me in K2 (which I had previously
sent to them). I specifically said not to freeze the samples; they shipped them
on dry ice! I was just doing one micron sectioning, bu
You wrote:
Probably the bone staining will be OK - how about sending the student to the
arctic circle for a few days in winter? - that may be a good lesson about
not freezing tissues. Just kidding :-)
On Fri, May 20, 2011 at 9:24 AM, Tora Bardal http://lists.utsouthwestern.edu/mailman/listinfo
All very insightful input. I took the liberty of forwarding some of the
comments to the client, I think I errantly sent some of the critical
comments about him too. I'll probably hear about it later, the best was the
clown residency, I was in tears laughing. i'll let you all know what he says
about
And the block in question has already been proven positive using THAT procedure
and antibody during validation.
Claire
From: histonet-boun...@lists.utsouthwestern.edu on behalf of Thomas Jasper
Sent: Thu 5/19/2011 2:39 PM
To: pete.peder...@healthonecares.com; h
The bast approach will be to try to do the cartilage/bone staining. It is
preferable to do this than to consider the samples already ruined and incur in
the expense of a new sampling.
René J.
From: Tora Bardal
To: histonet@lists.utsouthwestern.edu
Sent: Friday, May 20, 2011 3:24 AM
Subject: [Hi
Q#1 - yes, it is still 200 proof
Q#2 - no it should be not considered as a controlled substance. If you still
have to keep a log of its use = bureaucratic ignorance and lack of
adaptability, like the case in the British army were the artillery kept in the
personnel assigned to each movable gun 2
Tj,
Amen brother!
GD
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper
Sent: Thursday, May 19, 2011 2:39 PM
To: pete.peder...@healthonecares.com; histonet@lists.utsouthwestern.edu
Subject: RE:
Pete,
OK, time for an example:
A pathologist orders 4 IHC's on a block.
I run 5 slides total: 4 IHC slides with a section of patient tissue and a
known positive. 1 slide with patient tissue only for the negative control.
The one negative control is put through the retrieval/protocol that is
Histology Colleagues,
I am interested in collecting information to better understand what motivates
Histotechnologists in the workplace as well as job satisfaction levels within
our field. This ten question survey should just take a few minutes to complete
and survey responses will be kept
Probably the bone staining will be OK - how about sending the student to the
arctic circle for a few days in winter? - that may be a good lesson about
not freezing tissues. Just kidding :-)
On Fri, May 20, 2011 at 9:24 AM, Tora Bardal wrote:
> One of our students accidentally put his samples in t
One of our students accidentally put his samples in the freezer after
formaldehyde/glutardialdehyde/PBS fixation. The samples (fish very early
stage, of course rare species) were meant for LM/EM morphology studies.
Good morphology is out, but is there a chance he can do cartilage/bone
staining?
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