Any chance it should have been 0.5 g lithium carbonate in 500 mL water?
Maybe someone typed the label wrong? That's happened to us before. Is it
supposed to be a 1.0% solution, or a 0.1% solution?
Only reason I'm asking is that the only place we use lithium carbonate is in
the LFB stain for
Hi Lydia,
I think Tony Henwood has it exactly right in talking of DAB intensification.
The article he sites and several others show how much more sensitive DAB
intensification can make an ordinary iron reaction. And you are not looking in
bone marrow or spleen but somewhere where there
Patsy,
I have used this reprocessing schedule from Lee Luna's book Histopathologic
Methods and Color Atlas of Specials Stains and Tissue Artifact. This procedure
has worked for me on a couple of occasions, You may need to change the times a
little since your tissue samples are so small. Luna's
Patsy
I have used a technique similar to the one in this article when I was fortunate
to work on some mummified tissue, with surprising success, when I was back in
Glasgow many, many moons ago
Mekota A-M, Vermehren MDetermination of optimal rehydration, fixation and
staining methods for
Hi: Would anyone have a hereditary nonpolyposis colorectal cancer control,
(for MHL1, MSH2, MSH6) to share?
Thanks in advance,
--
Marian L. Powers
*Doctors Pathology Services * **
c| 302.747.0580
o| 302.677. ext: 110
f | 302.677.0010
___
Just wondering what y'alls opinion is on validation: I don't really understand
why optimization isn't enough. At that point, the pathologist has said what he
wanted the stain to look like, so why do 3-10 positive slides on the new
instrument to compare to previous slides from another
Here is a link to a CAP presentation from their website, maybe this will help
out. http://www.cap.org/apps/docs/education/lapaudio/pdf/051910_pres.pdf
Joelle Weaver MAOM, BA, (HTL) ASCP
http://www.linkedin.com/in/joelleweaver
From: amber.mcken...@gastrodocs.net
To:
Optimizing is getting the stain to look a certain way based on retrieval,
incubations times etc validation is the process of running cases that are
known positives of varying levels(degrees of positivity) and running them with
normal cases to ensure the antibody is staining as intended
Amber,
Optimizing the antibody is the first step of validation. Running the new
protocol against previously stained cases is the second step and shows you are
getting results as good as, if not better, with the new vs the old.
Cindi Robinson HT(ASCP)
Mercy Medical Center
Dunes Medical
Amber
When you optimize a stain you are normally just looking at one tissue type or
possible a couple, not sure how you handle protocol development. The amount of
work that is required for validation in the clinical setting is dependent upon
the type of primary antibody that you are using.
Hello Histoland,
We are seeing unusually large volumes of fall out in our paraffin lately.
The precipitate is almost muddy or oily in appearance. We noticed it
immediately as it was melting, so there was no way anything else had been
introduced to cause this. As a result, we are seeing knife
Greetings Histoland. We went live with Soft in September, and are still having
issues finding a slide label/ribbon combination that doesn't fade or smear.
Also, the slide labels currently being generated will not scan for our
Pathologists. The products we are currently using are from a
Amber, is this new instrument the same kind as your old instrument and are you
using the same reagents? Or is it a totally different platform with different
reagents?
Putting in a new instrument of the same kind and using the same reagents just
requires verifying the new instrument works. You
Anyone had success getting Calmodulin up and running? I'm running in to some
difficulties with an Abcam antibody one of our researchers gave me.
Thanks for any assistance
Ronnie
Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
Hi,
I use labels and ribbons from this company. They do not wash off or fade.
http://www.barcode-labels.com/solutions/laboratory-barcode-systems
Labels part# 92169 and ribbon #T84252074
Paula K. Pierce, HTL(ASCP)HT
President
Excalibur Pathology, Inc.
8901 S. Santa Fe, Suite G
Oklahoma City,
The Turnbull's blue method as you describe it will not detect iron in tissues.
Potassium ferricyanide will give a blue precipitate only with iron(II)
(ferrous). In tissues, the iron is present as iron(III) (ferric) in such
proteins as ferritin and haemosiderin. The iron of
Can someone recommend the best clone for Retinoblastoma Gene Protein on
formalin-fixed paraffin sections?
Thanks
Ronnie
Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com
700 Children's Drive
Columbus, OH 43205
Hello everybody,
Is anybody out there using bar code system for slides/cassettes?
My supervisor asked me to find out pros and cons and more information about
this. I have no idea where to start...
Thank you in advance!
Leticia Figliuolo
___
Histonet
Leticia,
We used Ventana Vantage in my lab and loved everything about it. It is so much
more than just a labeling system. Check it out. It is pricy but worth every
penny in what you will gain in productivity and error reduction.
Jan Mahoney
Omaha, NE
From: leticia.figliu...@roche.com
Seems the actual amount of validation slides can vary with different Medical
directors( Pathologist) or thats what Ive seen in places. They( The
Pathologist) sign off on it. With that said ER/PR and Her2 have more vigorous
criteria( very specific, such as the 25-50). Also, the 2011 AP133
Hi all,
Dako also offers a labeling system. So does Cerner and Fisher diag. There are a
few others out there too
Just thought you should know there are other less expensive systems out there
that might provide you with what you are looking for thats not as expensive. It
really depends on
Maybe you got a bad lot that is contaminated causing the knife marks? Also, Ive
noticed that if you let your paraffin get to hot, it breaks down and that could
be one reason for a consistency issue. Have you changed anything at all
recently to your process? Hope this was helpful.
Kim
Does anyone have recommendations for a good vendor for H.Pylori for IHC? We
have tried many, but our pathologists complain that they are not specific for
H.Pylori and that all gram negative bacteria are staining. Currently we are
not running this but our pathologists are interested in it.
We use the Rabbit Polyclonal H. pylori from Cell Marque on the Ventana
Ultra's... We're a natinowide reference laboratory and our clients are all
very happy with the specificty of the staining. Let me know if there's any
more information you need.
Drew
On Wed, Nov 16, 2011 at 17:15,
We use Ventana's, manufactured by Cell Marque. Most, if not all, of out
pathologists have opted for this instead of histochemical staining for
HP.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Kim wrote: Seems the actual amount of validation slides can vary with
different Medical directors( Pathologist)
Yes, validation is simply the process of proving to yourself and anyone else
(pathologist, clinician, inspector, patient, lawyer) that the product works as
intended. Medical
Hello Histonets,
I have some questions about paraffin slides storage.
1) For how long do you store paraffin slides after sectioning ?
2) At what conditions do you store the slides ?
Itai
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
27 matches
Mail list logo
