I am out of the office until 07/16/20= 12.
I = will respond to your message when I return.
Note: This is an automated response to your message quo t;Histonet
Digest, Vol 104, Issue 10 sent on 7/9/2012 1:00:01 PM.
=
This is the only
For the paranoid out there: imagine what a lawyer will do to a hospital
in court when Formalin (or other proper fixative) was not allowed to be
utilized and the specimen is ruined. The lawsuit settlement will make a
fine from OSHA look like buying a candy bar. More than likely it will
be a
Hi Histonet members, I am in trouble. I need 1 mm. tissue array punch set (
Beecher manual instrument ). Either you can sell us one or exchange for a box
of ten sets of 0.6mm size punch sets. Please contact me at
sayeedud...@sbcglobal.net
Thanks in advance for your response.
Sayeed.
What are you using for Endogenous blocking? If you are using methanol with H2O2
that may be your problem. Many CD antibodies are very sensitive to the
methanol. Try PBS or whatever buffer you are using to dilute the H202. I would
also try going straight form the -20 into cold acetone without
I was just told by my Biocare rep that today was the last day they are selling
P63 antibody. I would like to purchase IVD P63 from another vendor, who else
sells it? I have having a few problems finding good info just using Google.
What is your lab using for diagnostic purposes?
Cathy
Greetings!
I want to stain for human vimentin in mouse tissues (human fibroblasts). I
many years ago, I used a biotinylated V9 clone for just this purpose and it
worked great. I tried the biotiylated V9 clone from abcam, but I am not
getting any staining at all. Any suggestions?
Kim
Ventana has gotten the sole patton for that antibody now I beleive, or will
have soon.
From: Cathy Crumpton cathy.crump...@tuality.org
To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Sent: Tuesday, July 10, 2012 11:51 AM
Subject:
I just spoke with my Biocare rep. He said it is untrue at this time, and they
are still selling p63.
Clare J. Thornton, HTL(ASCP),QIHC
Assistant Histology Supervisor
Dahl-Chase Diagnostic Services
417 State Street, Suite 540
Bangor, ME 04401
cthorn...@dahlchase.com
-Original Message-
Hi everyone,
Can someone refresh/enlighten me as to why Methanol is used in H2O2 blocking.
Let's assume that I take my slides through the xylene/ethanol steps and that
after the 70% etoh wash step, they are now in TBST.
I could see adding methanol if you were doing the H2O2 blocking as part of
If you have large specimens, it is best to open them as soon as possible. If
it is a breast, at least do your prelim measurements and slice it open, leaving
it still attached, so that the formain can penetrate the tissue. If you go
ahead and slice it up, at least wrap it, in order with paper
Histonet gurus,
Why is each microscope ocular marked and operated differently? For instance the
right one has a knurled focusing ring, is easily focused and has detailed
graduations while the left one is not really set up to focus quickly and has
only minimal graduations? Always wondered
Clare,
Just got off the phone with my Biocare rep, Lissa Wall. I'm afraid that it IS
true. Long story short, legal issues going on, and Biocare will stop selling
P63 TODAY, 7/10/12.
Please talk to your rep ASAP to get those last minute orders in today.
I well remember when the last (no name)
Yes, it is true. I just talked with Biogenex Laboratories and they will be
carrying both the 4A4 and 4B1E12 clones in the foreseeable future. Other
places I have talked to are depleting their stock and not carrying P63 anymore.
Ventana wants $800+ dollars for 50 tests worth and do not carry
Tim, etal:
This is easily understood: focusing and setup work the same on binoculars,
one eyepiece is focused with the main focusing system, the other is used to
match
focusing with both eyes. First focus the scope (binoculars or microscope)
thru
the simple (non focusing) eyepiece, then use
Thanks Peggy,
That is clear. I used microscopes for years with one fixed ocular and one
focusable ocular. I was wondering about why now both oculars are focusable
yet one has more usability than the other. Maybe to accomodate greater
variation? Or maybe is due to the advent of parfocal
I teach immunology and histology to biomedical laboratory technology students
and I would like to perform a simple direct immunofluorescence technique on
normal animal tissue obtained from the butcher. I believe I could easily
obtain either kidney, heart or liver either from pig or beef.
Tim,
You are welcome, but I'm not Peggy. I'm Lee, her husband.
I'm very familiar with binoculars, so I only assumed that they are the same
as microscopes.
Lee Wenk
-Original Message-
From: Morken, Timothy
Sent: Tuesday, July 10, 2012 2:49 PM
To: Lee Peggy Wenk ; Histonet
Subject:
Tim,
The duel-adjustable eyepiece vs the single adjustable one, I believe has
to do with the type of prism that is used.
Mis-matched eyepieces can be a problem, especially if from different
manufacturers or even magnification. Magnification differences would be
somewhat obvious as you will
Dear All,
My microscope training has been mainly on brightfield microscopes using Koehler
illumination.
Recently, in discussions with Veterinary Pathologists, they have been
advocating winding the condenser way down to the field diaphragm when they are
examining urine samples. This goes against
Hi Liette,
I didnt see if you got help yet. Ill give it a try. First I
would go with the pig kidney,. and this antibody looks like it should work .
http://www.abcam.com/Pig-IgG-secondary-antibody-H-L-ab6911.html
This is my best guess off the top of my head. If you try it and
Hi Laurie,
How are you?
The idea of pushing out of Koehler as you described gives you a poor-man's,
pseudo-phase effect.
I use it for unstained frozen sections of renal and skin biopsies that are
destined for immunofluorescence.
And then I get mean and request the students who I have
Why do some people use methanolic H2O2?
Why do people rehydrate after dewaxing?
Both are unnecessary, under usual conditions.
Methanol was used in the early days as a peroxidase blocker by itself.
The combination was devised as a belt and bracer method.
As you stated, you use aq H2O2 effectively.
Hi Carl,
What do you mean by Why do people rehydrate after dewaxing? Do you really
mean that slides do not require rehydration or do you mean that slides can be
left to dry after de-waxing prior to staining.
Re-hydration is necessary, otherwise xylene will prevent aqueous stains from
doing
23 matches
Mail list logo
