Hello,
I have noticed that our biotinylated secondary antibodies on occasion cause
nuclear staining in some samples. Why is this? It is not every time so I
find it rather stange. Anyone know why this is happening and what I can do
to avoid it?
Thank you for any suggestion,
Eva Permaul
Georgetown
Check with your local Leica rep.
Beatrice DeBrosse-Serra HT(ASCP)QIHC
Isis Pharmaceuticals
Antisense Drug Discovery
2855 Gazelle Ct.
Carlsbad, CA 92010
760-603-2371
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On
Yes it fits on the microtome where the permanent blades go for older
microtomes that do not have disposable blade holding stages.
Patsy Ruegg, HT(ASCP)QIHC
Director of Histology and IHC
IHCtech a subsidiary of Flagship Bio-Sciences, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
For the last couple of years, I've thought this was true, but I didn't have the
guts to say so. Thank you, Richard for bringing the truth out and getting it
accepted.
Allen A. Smith, Ph.D.
Professor of Anatomy
Barry University School of Podiatric Medicine
Miami Shores, FL
-Original
Hi everyone I am having a problem with the fast green counterstain for the PAS
for fungus. The fast green stain is simply not staining anymore no matter how
long I leave it on the specimen. Do you have any suggestions on how to correct
this or perhaps recommendations for a different
Prepare a fresh solution
René J.
From: Michele Carr michelecar...@yahoo.com
To: Histonet@lists.utsouthwestern.edu Histonet@lists.utsouthwestern.edu
Sent: Monday, July 23, 2012 12:11 PM
Subject: [Histonet] counter stain for PAS for fungus
Hi everyone I am having
I have used a working solution light green Sf yellowish C,I. # 42095 and
the only time I had problems was when someone left out the glacial acetic
acid in the stock,
Stock is light green SF yellowish 0.2 gm,distilled water 100.0 ml, 0.2 ml
glacial acetic acid.
Working is 10.0 ml of stock, 50.0
Rena,
If one uses Light Green (SF Yellowish) as a counterstain, it is important to
monitor it closely. That solution will grow fungus and will show up as an
artifact on stained slides. It is easy to tell the source because it is not
stained, whereas your silver or PAS techniques will stain the
Hello all!
We are budgeted to get an automated coverslipper for our department.
When we talked about it with our service rep, he asked if we wanted a
glass or tape coverslipper. I worked with a glass one many years ago
when they first came out and all I remember is breaking and sticking
All,
We are looking for a site to send our NEURO Electron Microscopic blocks for
EM ONLY (Tech only). There would be about 25-30 cases a year (muscle, nerve
and possibly brain). If you have information please let me know. I would
appreciate it.
Patricia Karlisch
Supervisor,
Try Hopkins or Univ of Maryland Medical Center.
Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 126
(All Deliveries to Suite 127)
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
wben...@cua.md
I'd love to know too. We also use a xylene substitute.
Claire
From: histonet-boun...@lists.utsouthwestern.edu on behalf of Brendal Finlay
Sent: Mon 7/23/2012 12:57 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)
Hello all!
We are
Hi All,
I have a PA that uses acetic alcohol for lymph nodes. I have tried all of the
premade ones and he doesn't like any of them, so we are back to making it
again. How do you determine expiration dates of solutions made in house?
Thanks,
Susan Baker
The
It all depends on how long you want to store the slides. The tape has a
tendency to come off, whereas the glass slides, if covered properly, can be
stored indefinitely. The glass coverslippers have improved quite a bit, and
there are several vendors out there who have them. We have one here,
We believe it has to be the secondary as we have nuclear staining in the
absence of primary antibody.
Eva
On Mon, Jul 23, 2012 at 12:58 PM, ihcs-wojcieszyn i...@smithsys.net wrote:
Hello,
Please explain how you know that it is the secondary and not the
primary? We Have confirmed that
Add some acetic acid to the fast green.
On Jul 23, 2012, at 9:11 AM, Michele Carr michelecar...@yahoo.com wrote:
Hi everyone I am having a problem with the fast green counterstain for the
PAS for fungus. The fast green stain is simply not staining anymore no
matter how long I leave it
Michelle -
I agree that the light green is dead and you should make some new or buy a
commercially made solution. However, if you want to try a different
counterstain try a light hematoylin (usually used for glycogen) or metanil
yellow. Either will give good contrast to the pink of the PAS.
It is possible that this is due to Biotin nuclei where excess biotin is found
in the nuclei of some cells, see below:
Optically clear nuclei have been reported in endometrial epithelium associated
with first and second trimester abortions (Sickel di Sant'Agnese 1994).
Optically clear nuclei
I should have added that this was from the workshop notes on a Hypotheticals
Workshop I ran last year at our Australian National Meeting.
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Laboratory Manager Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
Hi All does any one has procedure for making inhouse ZN control if they can
share with me . I will appreciate itthanksNaveeda
--- On Mon, 7/23/12, histonet-requ...@lists.utsouthwestern.edu
histonet-requ...@lists.utsouthwestern.edu wrote:
From: histonet-requ...@lists.utsouthwestern.edu
Hi - all hands down the film. Faster, less daily issues. I was fortunate to
be one if the first to try this in the Boston area in the early 90's. I have
done numerous studies including long term storage. You need to ensure adequate
'clean' xylene drips 3-5 per slide. GOOD LUCK
Candace
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