[Histonet] RE: MOHS IPs

Hello Karen, Back in the old days of doing IHC manually on mostly fresh, frozen tissue I would immediately place the slide with the frozen section into a coplin jar of acetone to fix it. Keep the coplin jar of acetone in the cryostat. Leave it in the acetone for only 10 minutes. Try

RE: [Histonet] RE: MOHS IPs

I agree with Barbara try fixing in cold acetone/ethanol mix even = if just for a couple of minutes then go directly to buffer do not dry after= fix, I would dilute the protease 1:4 with buffer (PBS or TBS what ever u u= se) and do it for a short time. Enzyme digestion on

RE: [Histonet] RE: MOHS IPs

Thanks for the reply, We are pre-fixing in a formalin substitute, but we'll give the acetone a try. I have diluted the protease, but have not gotten any good results. We'll keep trying with that as well. We've tried multiple incubation times for the cytokeratin... From 5 minutes all the way

RE: [Histonet] thawing and refreezing muscle?

I have tried this method as well and it works just as described below. Sarah E. Lewis HT, ASCP The Research Institute at Nationwide Children's Hospital Center for Gene Therapy Neuromuscular Division Rm WA3110 (614)722-2204 -Original Message- From: Tony Reilly

[Histonet] Re: MOHS IPs

Hi Karen, It could be that your cytokeratin antibody only recognizes a formalin fixed epitope conformation and enzyme digestion has nothing to do with it. Also try fixing in formalin and then try the stain again with and without the diluted enzyme digestion. Brief antigen retrieval (Citrate

[Histonet] Shipping to Countries Outside of the US

Hello All, A question was posed to me by our Cancer Resource Department... I am wondering if you/lab has had experience with shipping tissue specimens outside the country. We have a study where we may be doing this and they are asking if we have to have a special permit to ship outside our

[Histonet] Gram stain

Hi All, Does anybody have a protocol for Gram stain on FFPE mouse intestine sections. We are looking to distinguish between Klebsiella and Vancomycin Resistant Enterococcus (VRE). I would like to avoid using Picric acid as some protocols suggest. Thanks in advance.

[Histonet] Benchmark Ultra troubles

Hi Ventana-users, we have a special technical problem with our Ultra. It seems, that the liquid in the waste-tube is sometime pressed out of the hole on the platform like a fountain. You can imagine the nice surprise, when the oil drops from the Ultra-roof onto the carousel-top. Everything is

Re: [Histonet] RE: MOHS IPs

What detection kit are you using? Sent from my iPhone On Oct 2, 2013, at 10:47 AM, Bauer, Karen L. bauer.ka...@mayo.edu wrote: Thanks for the reply, We are pre-fixing in a formalin substitute, but we'll give the acetone a try. I have diluted the protease, but have not gotten any good

Re: [Histonet] IHC turnaround time

In less than 8 hours I can get 96 Ihc stains done with our dako link and that is two runs Sent from my iPhone On Oct 1, 2013, at 1:15 PM, McKenzie, Emily mckenzie.em...@mhsil.com wrote: Hello all, I am currently working on a Six Sigma Green Belt project and am looking for some data. Can

Re: [Histonet] Gram stain

We have had great success with the Twort gram stain. From: Mesru T turke...@gmail.com To: histonet@lists.utsouthwestern.edu Date: 10/02/2013 10:13 AM Subject:[Histonet] Gram stain Sent by:histonet-boun...@lists.utsouthwestern.edu Hi All, Does anybody have a

[Histonet] Hi

Hi Im looking for an acid phosphatase enzyme procedure that might be out there the one we tried in the past was difficult to work up and get good reproducible results. Thanks Helene D Upstate University Medical Syracuse, NY ___ Histonet mailing

[Histonet] RE: Validating new Benchmark instrument

I am thinking there has to be a way to integrate the Validation into the Optimization the vendor does. I think you should be able to make microarray blocks/slides of at least 10 positive and more for ERPR per CAP during the optimization. Now, the vendor will say they only do Optimization, but

[Histonet] need lever clamp for blade of leica microtome

Does anyone have an extra lever clamp for a Leica microtome? I'd be happy to pay for it, and of course, for shipping! Thanks, Patty Lott UAB CMBD Core Lab 205-934-2007 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

Re: [Histonet] Shipping to Countries Outside of the US

According to our Receiving department, each country/institution has its own permit requirements for receiving samples from outside of their own country. It is up to the shipper to inquire of the receiver as to what permits are needed to ship to that country (Germany, in your case). My source

[Histonet] RE: Histotechnologist, (ASCP, QIHC certified) and Pathology assistant position in Seattle area

Needing a Histotechnologist (ASCP, QIHC certified) or Scientist (Histopathology) for a Seattle area. Five years of experience in routine histology including special stains, IHC and pathology knowledge. Send an updated resume to yli...@numirabio.com to be considered. A full job description

[Histonet] Vacuum and pressure in tissue processing

Dear friends, I recall hearing at a conference (or maybe it was just a casual conversation by an expert during a NSH symposium break) that vacuum and pressure in tissue processing really accomplishes very little. I do believe that using heat and agitation of the solutions provides more

[Histonet] Trivew

Please share protocol for Triview from Zetta Corporation for Venatna. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Inconsistent Sections with Cryostat

Hello All, I have a Leica Cryocut 1800 with a Reichert-Jung 2020 microtome that has sat for 8 years and went through a move from one academic building to another. I am trying to section brook trout brain, but am having difficulty getting consistent sections. I will get a good section and then