Good Morning,
I wanted to share with everyone the Florida Society of Histotechnology's Fall
newsletter. We have a great article on immunohistochemistry that will allow you
to get 1 CEU credit if you submit your answers to our Continuing Education
Chairperson. If you are a FSH member it is free
Histonetters:
Our lab needs to paraffin embed and cut bone. Is there a special
process for fixation of bone, or can it be harvested and dropped right
into NBF?
--
Nicole Cosenza
Research Technician
Institute for Plastic Surgery
SIU School of Medicine
Springfield, Il
217.545.3862
Yes 1:20 dilution
Terra Wineman, HTL (ASCP)CM
Research Biologist, Nutritional Physiology
636-926-7476 phone
terra.wine...@novusint.com
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nicole Cosenza
Sent:
Good morning,
We are currently searching for a histology professional to join one of our
top clients. The position will be a field based support specialist and will
cover New England. The position offers a very competitive salary, bonus,
company car, gas card, cell phone, and laptop.
The
Nicole,
You can very easily fix the bone in 10% NBF and then go into your
decalcification process. Remember that fixation rate of bone is generally
around 1mm per 24 hours (in all directions) and that it is good to have a
minimum of 20:1 ratio of solutions to specimen size for each step. I
Sorry Nicole I forgot to finish a thought before I hit send. What I was going
to say was any additional information you could provide regarding the specimen
and what you wanted to see at the microscope can sometime yield additional
information regarding specific processing protocols, staining,
Greetings,
Our lab recently got a new cryostat (a cryostar NX50). It sees light to
moderate usage (3 - 20 hours a week) and has been operational for about 3
weeks. It has had a host of problems since we received it (shipped with a
busted motor and the sensor in the window did not detect
Greetings,
Has anyone received their credits for the California Society for
Histotechnology meeting which took place this May 2014, in Woodland Hills.
I know a few people who are still missing their credits and some need
paperwork for reimbursements.
Thanks,
Maria Samaan
Hi Nicole,
There are various ways to fix bone. My lab generally fixes mouse hind limbs
with 10% Neutral Buffered Formalin for 3 day with or without perfusion. The key
to fixation is proper grossing and long enough time for fixative to penetrate
tissue.
Please feel free to contact me with any
I have not receive the certificates for the CSH back in May. I asked several
time for the CE because I have many employees that needs it for reimbursement.
In our institution, we only have 3 months to submit the documentation so all of
us did not get the reimbursement and I don't know if
Does anyone use a cold plate, like that used for embedding, for icing blocks
for sectioning? Just an idea
Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies
UC San Francisco Medical Center
Box 1656
505 Parnassus Ave
San Francisco, CA 94143
USA
We have a brain fixing with phenol formalin, any suggestions on anything
special we need to do with the tissue before processing?
Tanya G. Abbott RT (CSMLS)
Manager Technologist, Histology/Cytology
St. Joseph Medical Center
Reading, PA 19603-0316
ph 610-378-2635
fax 610-898-5871
email:
I had an employer years ago who used this. The disadvantage that bothered me
the most was the paraffin shavings flying around due to lack of moisture. It
created a very dirty environment, and there were frequent floaters due to the
difficulty in keeping the microtome blade clean. I wouldn't
Hi Tim,
We had looked at one time, for a smaller cold plate to be used at the microtome
during sectioning and could not find anything suitable. We were told that they
would be very expensive and the one example I found was just that. It would
eliminate the water and mess of the melting ice
I have an old cold plate from an old embedding center that one of my techs
uses. I do wish someone would make a reasonably sized/priced one for individual
use.
Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org
www.saintjosephsatlanta.org
5665
We are a small lab and we each use a cold plate from the embedding station.
Our microtomes sit on either side of the embedding station and each side has a
cold plate.
Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way |
How do others divide up the work in the department? Do you have techs on like
a weekly schedule to cut , immunos, do gross, log specimens in, change
machines? Just wondering how others did it. Thanks
Anita Dudley
Providence Hospital
Mobile alabama
Hi Matt,
This sounds like a great opportunity...
Can you please provide more details about the position?
I have 20 years of Histology experience and would like to continue to
further my career in the field...
Thank you,
Nadine Pizzella
On Nov 3, 2014 10:56 AM, Matt Ward m...@personifysearch.com
I found a stainless steel pan measuring about 14 long, 9 wide and 5 deep and
filled it with DH2O. I got it out of the freezer when I arrived at work and by
the time I was ready to face, there was a bit of melt on top of the ice block
that was just right. Adding a bit more DH20 at the end of
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