[Histonet] Re. Decalcification with formic acid sodium

2015-07-28 Thread Tyrone Genade via Histonet
It isn't very clear, but for the Formic Acid/sodium Citrate buffer you have to use trisodium citrate. 10 g of disodium or monosodium citrate wouldn't work as well... -- Tyrone Genade Orange City, Iowa tel: (+1) 712 230 4101 http://tgenade.freeshell.org *

[Histonet] EMA/MUC1 IHC on dogs

2015-07-28 Thread Jan Shivers via Histonet
Does anyone perform EMA/MUC1 IHC on canine tissue? If so, what company is your vendor? You can reply to me in a private message. Thanks in advance. -- Jan Shivers Senior Scientist IHC/Histology Section Head Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333

Re: [Histonet] axolotl lymphatics

2015-07-28 Thread John Kiernan via Histonet
Instead of an antibody, you might consider enzyme activity histochemistry, which is much less expensive. Demonstration of 5-nucleotidase activity in the presence of levamisole detects lymphatic endothelium. Sections can also be stained for alkaline phosphatase activity in the endothelium of blo