Michele:Methodologically it is much more difficult to freeze/section formalin
fixed tissue so it is unnecessary adding an additional layer of difficulty to
the procedure pre-fixing in formalin.Although I have not tried it, there is no
effect of NBF on the amount of fat so there should not be a
Hi Edison- I have had some headaches getting the SOX-10 to run in the past but
I now have all of the kinks ironed out and it works quite well. I had started
with the CellMarque mouse monoclonal of their RTU SOX-10. I had it working
well and then Leica recalled their red detection kits. I
What kind of processor are you using?
On Tue, Jan 26, 2016 at 1:36 PM, Jennifer MacDonald via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> There are a couple of things that it might be.
> 1. Uneven deparaffinization before staining.
> 2. Water in your last reagents/paraffin on the
Hi Edison. I use the CM SOX-10 polyclonal RTU on my Leica Bond-Max(s). I also
run it with Leica's red kit. I have had zero issues. I do use CM's SOX-10
controls though, they seem to stain the best for me. My protocol is ER2 for 30
minutes at 97 degrees. I run them down from 100%-SUB-X and have
Hello,
Did you call the vendor/manufacturer of your chemicals? Sometimes, if they
have to dispense the chemicals from a different place it can cause issues. I
had that happen with reagent alcohols.
Good luck,
Linda Dee, BGS, HT(ASCP)
From: Jay Lundgren via Histonet
Good Afternoon,
We would like to invite all of you to Branson Missouri for the first Joint
Meeting of Missouri/Arkansas Histology Societies. We will be at the Radisson
Hotel right in the middle of everything with the program below. If you have any
questions please contact either Sharon
This looks wonderful!
Be sure to keep me on your list Pam so I can consider this meeting!
C
On Thu, Jan 28, 2016 at 2:50 PM, Pamela Marcum via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
>
>
> Good Afternoon,
>
>
>
>
> We would like to invite all of you to Branson Missouri for the
Does anyone use Klinipath Coverslipping Tape and Sakura Coverslipping Tape?
Is there any issues comparing the Sakura Coverslipping Film?
Best Regards
___
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Histonet@lists.utsouthwestern.edu
Dear All,
I need your guidance in the following issues,
Issue No:1I am going to identify two markers on a single cell (macrophage) as
well as on two different cells (CD4 and CD8) in a single tissue (costaining).
What could be the best secondary conjugate systems for detection in this case
(I
Hello,
Anybody running Cellmarque's RTU SOX 10 antibody on the bond III, any protocol
suggestions, I would like to run this antibody with Leica's red kit.
Thank you
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