[Histonet] Attention Daniel Blackburn about plastics embedding

2016-07-19 Thread Gayle Callis via Histonet
You wrote: My lab hopes to get into plastic sectioning. We need to be able to process tissue pieces as large and thick as possible, but see that the largest embedding molds for JB4 are only 13x19mm by 5mm deep. We have two questions: (1) Do any of the available media (plastic or resins)

Re: [Histonet] glycol methylacrylate GMA enzyme and IHC

2016-07-19 Thread Gayle Callis via Histonet
You wrote: Hi all, I am going to be exploring some tissue embedding using Glycolmethacrylate (GMA), as I read that it can preserve enzyme function in some cases and potentially can be used for IHC. However, information is a bit scant and can be contradictory at times, to say the least.

[Histonet] Low temperature freezer

2016-07-19 Thread Tamara Howard via Histonet
Richard - We learned last year (the hard way) that chest freezers are more likely to survive a flood than are uprights, if that is any help! The compressors tend to be near the top of the unit in the chest freezers. I think the footprint may be the main deciding factor for many labs; we just

Re: [Histonet] Prefilled formalin containers

2016-07-19 Thread Kienitz, Kari via Histonet
I have had this annoying experience a few times over the last 20+ years. It seems to me the breakdown is with quality control at the manufacturer. Most recently, 1 out of 5 containers came back to the lab leaking. I put pressure on my vendor, who put pressure on his vendor. Unfortunatly,

Re: [Histonet] expiration dates

2016-07-19 Thread Elizabeth Chlipala via Histonet
Linda We have in our reagent policy that any chemical that does not come with an expiration date - we assign it a 5 year from receipt expiration date. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax

Re: [Histonet] expiration dates

2016-07-19 Thread Bell, Lynne via Histonet
My can of Fisherbrand Freeze'it does not have an expiration date on it but I have Cytocool II from Richard-Allan Scientific does have one (it's printed on the bottom of the can). Lynne Bell, HT (ASCP) Histology Team Leader Central Vermont Medical Center 130 Fisher Road Berlin, VT 05641

[Histonet] expiration dates

2016-07-19 Thread Blazek, Linda via Histonet
Does anyone see an expiration date printed on their can of Freeze Spray? I was told by the company I purchase mine from that it was not needed because it was "not a medical device". Thanks Linda ___ Histonet mailing list

[Histonet] Blog Post (with Explanation)

2016-07-19 Thread Lester Raff MD via Histonet
The linked blog is not about lab/histology, but a histonetter Downsize, Maybe subscriber who read it suggested that it could perhaps be useful for h. pylori and worth a post-so here goes. http://www.chicagonow.com/downsize-maybe/2016/07/we-take-a-shine-to-yinmn-its-the-new-blue/ Lester J.

[Histonet] Plastic embedding and Microtomy

2016-07-19 Thread Cassie P. Davis via Histonet
Hi Daniel, I haven't seen plastics embedding and microtomy since the mid 90's. When I was at CCHS in Newark, DE we used to do our bone marrow biopsies in them. At that time there were molds big enough to embed a penny in. Yes, you need a different microtome to section these