Ana, you should fix before sectioning. Chris Van der Loos did an excellent
study on loss of cytokines in frozen sections vs whole cells. See his paper:
Immunohistochemical Detection of Interferon-y, Fake or Fact? CM Van der Loos,
et.al., J Histochem Cytochem, 49(6):699-709, 2001. Www.jhc.org
Hi everyone,
Just wondering what is people's opinion and protocols for IHC in mouse frozen
sections targeting secreted proteins and cytokines. I see lots of places using
fresh frozen sections/snap-freeze and cold acetone or methanol/ethanol
post-fixation. Is this an issue when it comes to
