Hello all,
Can someone recommend an economical disposable blade for
grossing eye globes? Sharp enough to slice through sclera smoothly, strong
enough to finish the cut through the lens.
Cheers!
Sandy
Sandra J. Cheasty, HT (ASCP)
Histology & Necropsy Supervisor
UW-Madison, School
Renee, for practical purposes in histology they are pretty much the same. Note
that the original water quality is key because certain things can carry over in
each type of system. Commercial suppliers do all the filtering, conditioning
and finishing for you.
Deionized (DI) is now the usual
Going over checklist needed input on water quality. We are a small lab and use
distilled water purchased commercially. We do IHC but no special stains. We
use the water in our water baths and for preparing IHC buffer. Should we
upgrade to deionized or use distilled?
Renee H. Workman
Elaine, blocks last a very long time. The at the face the tissue may be
oxidized, like a section on a slide, but the deeper tissue should be good. If
you have several extra blocks just coat the face with paraffin to protect it.
However, the only way to be sure is to test them occasionally. If
Elaine
We have the temperature of our cryostat calibrated yearly by an outside
calibration company. If the thermometer you are using inside the cryostat has
been calibrated and is traceable I think you might have an issue with the
temperature reading that is being displayed on your cryostat.
Hi everyone,
Just want to put a question out there. . . .
How long can we keep control blocks (paraffin embedded tissue) before they go
bad? I just went through our old control blocks in our filing container and
some of our control blocks have dates as far back as 10 years ago. They have
Hello Histonetters,
I have several exciting opportunities for experienced Managers,
Supervisors and Lead Techs in hospital and private lab environments in
several locations throughout the US. These are some of the premier
employers in the United States. The positions are of course full time
Does anyone know any place that will buy or I could donate our expired
antibodies? I just went through our refridgerator and cataloged all of the
antibodies that have expired. We were using some for teaching purposes with
the students who come through on rotations however due to budget needs we
Dear Participants in FASEB's Shared Research Resources Survey,
Today the Federation of American Societies for Experimental Biology (FASEB)
released the report "Maximizing Shared Research
