Re: [Histonet] FW: cutting a 5um frozen section from a 50um section of brain

I just made the aluminum temple from a 1/2 inch 6x6 piece on aluminum from Home Depot. With the sheet you will este,Italy make it look like a clock face using a sharpe. Why you want to do this is i found the aluminum plates have enough variance that if I did not always use the same side it

Re: [Histonet] FW: cutting a 5um frozen section from a 50um section of brain

Hi Loralei, I will see If I can find someone with a sliding microtome. Are you using OCT to block the 50 um section? How do you ensure the section stays flat while blocking/freezing? Thank you for your reply ! E-van From: Loralei Dewe [mailto:lld...@gmail.com] Sent: Friday, October 06, 2017

Re: [Histonet] FW: cutting a 5um frozen section from a 50um section of brain

Hi Robert, thank you for your reply. So you froze the section on the aluminum with the "clock face" and then put this inverted, in the Microm 560 mold? ours is an older unit that has been around and not all the parts came with it so I don't have the 90 degree block chuck. Is this the fixed

[Histonet] Developing a multiplex cocktail

Hello Histonetters, It has been years since I had to create a multiplex of a dual-antibody cocktail and for the life of me am having a brain block on the best way to go about this. Does anyone have or know of a procedure to make this type of cocktail? *Haley Huggins, HT (ASCP)cm* *Technical Lab

[Histonet] looking for method for fixing frozen tissue

Hi everyone, I was wondering if anyone can tell me the best way to formalin fix frozen tissue for processing into a paraffin block. I have tissue in a minus 80 freezer and would like to cut a piece from it to fix in formalin and process. I'm thinking that I could put the tissue into the

[Histonet] CORRECTION Re: 2017 Region 1 Annual Meeting October 28-29 Portland ME

Corrected email address cthorn...@dahlchase.com On Friday, October 6, 2017, 1:27:26 PM EDT, LitePath via Histonet wrote: Hosted by Maine Societyfor HistotechnologyHoliday Inn by the BayPortland ME For more info & registration contact: Clare

Re: [Histonet] FW: cutting a 5um frozen section from a 50um section of brain

HI Ms. Powell!! , Thanks for the reply. When we last all saw each other at the Georgia Histopalooza I expressed an interest in training on the cryostat with Cindy Baranowski. She was so nice and had me come over to the hospital back in June. That was really really nice of her! But now I can't

Re: [Histonet] FW: cutting a 5um frozen section from a 50um section of brain

Haley, Maybe ask Stephen directly. He is on histonet, and has a website: https://www.pathologyinnovations.com/ Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -Original

Re: [Histonet] FW: cutting a 5um frozen section from a 50um section of brain

I would also be interested in knowing this tip if anyone knows it. I will also have to check out that book. We only do cryostat sections at our lab. *Haley Huggins, HT (ASCP)cm* *Technical Lab Supervisor* *1050 Las Tablas Rd, Suite 14* *Templeton, CA 93465* *Office: 877-230-1518* On Fri, Oct 6,

[Histonet] 2017 Region 1 Annual Meeting October 28-29 Portland ME

Hosted by Maine Societyfor HistotechnologyHoliday Inn by the BayPortland ME For more info & registration contact: Clare thorntoncthorn...@dahlchase.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] FW: cutting a 5um frozen section from a 50um section of brain

Hi Histonet , I have been trying to cut a FLAT 4 to 10 um frozen section from 50um sections of brain. Every time I think I have the tissue flat the sections are never in the same plane. I found a wonderful book in pdf format(A Practical Guide to Frozen Section Techniques, Stephen R. Peters)

[Histonet] Job opportunity

Hello Histonetters, - Have you considered a move out of the lab and into the field? - Would you like to extend your reach to help many labs across the country achieve better patient outcomes? - Can you see yourself working for an innovative company whose primary mission is