Re: [Histonet] Modified GMS Protocols

2017-12-31 Thread Bob Richmond via Histonet
If you choose another oxidizing agent than CrO3 (such as KMnO4, potassium permanganate) it's up to you either to find literature to back you up, or to do the stain with appropriate controls for the fungus you're looking for. Freida Carson (citation below) found that periodic acid, commonly substit

Re: [Histonet] Modified GMS Protocols

2017-12-31 Thread Nipuna Weerasinghe via Histonet
Thanks for your reply Gudrun, I agree with your statements. If KMnO4 used as a replacement for CrO3, it should be used under much milder conditions (low temperature and less incubation time) than H6IO5 as KMnO4 is a stronger oxidant. However, my question was due to the fact that KMnO4 was a comm