Re: [Histonet] Sakura glas2

We use Permount and an aliphatic hydrocarbon clearing reagent. Jennifer Get Outlook for iOS From: Bitting, Angela K. via Histonet Sent: Wednesday, July 18, 2018 8:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sakura glas2

Re: [Histonet] Hazy/cooked cells in H

Have you checked the processors? It is easy to “mix up” the reagents. Has tissue collection changed? Are the specimens left out longer or placed on paper? Do you use sponges? Any chance the fixation time has been decreased? Sent from my iPhone > On Jul 18, 2018, at 3:46 PM, Mac Donald,

Re: [Histonet] Hazy/cooked cells in H

Is anyone using freezing spray? We had this problem and all cases were traced back to the same microtomist. She used the freezing spray excessively and was causing “freezer burn” to the GI biopsies. Jennifer Get Outlook for iOS From:

[Histonet] Hazy/cooked cells in H

Our pathologists have said that we have had a few cases the past few days that are unreadable. They say the cells appear "cooked". Nothing in our process has changed, we have been using the same lot of reagents since the middle of June, and no errors were reported in our processing runs. Do

Re: [Histonet] New Microtome

We are using a Leica 2235 and love it. Manual or Auto mode, the control panel is on a moveable pad, so can be used anywhere. It has a foot pedal if you like that too! The techs took to it like a duck to water and it cuts like butter! Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor

Re: [Histonet] Microtomes

We have three Thermo 355S's.  They are nice because you can use them in the automated mode or manual mode.  All of our HT's use the manual mode, so now we just buy the manual version. Laurie Redmond HT (ASCP) Path MD -Original Message- From: Piche, Jessica via Histonet To: histonet

[Histonet] Microtomes

Good Morning Everyone, We are in the market for new microtomes and was wondering what everyone is using out there? We are looking for semi-automated/automated microtomes. We are demoing the Leica Autocut and the Thermo 355S. I really like the Leica microtome but am not crazy about the control