Hi Sandra,
>From your post, it looks to me like you are assuming the recommended
dilution should work. I apologize in advance if I have misinterpreted your
post in this regard.
When starting with a new antibody (including a new vendor for same Ab
and/or a new clone), do a range of dilutions that
Hi Sandra,
Being a mouse monoclonal, it may not cross-react with canines/felines; the
epitope may be specific to human follicular cells only.
The polyclonal Thyroglobulin from Dako/Agilent works fine on all the
mammals I've tried it on so far (and a frog as well).
Regards,
Jan
Jan Shivers
Hi Lisa,
I just worked up an antibody from abcam; cat. # ab85554
Rabbit polyclonal
1:1200 (0.6ug/ml) -1:1600 (0.4 ug/ml)
Heat retrieval in pH 6.0 solution
45 min primary Ab, 45 min secondary Ab (polymer system), 15 min chromogen
(AEC+)
Regards,
Jan
Jan Shivers
Senior Scientist
IHC/Histology
Hello,
We had to switch sources (to Novus) for Thyroglobulin
TGB04+TGB05 and cannot get it to work in canines or felines. Data sheet
suggests 1:100, pH6 citrate antigen retrieval. We've also tried zero AR and pH9
EDTA AR, to no avail. Any suggestions?
Thanks!
Sandy
Sandra J.
Our CgA has stopped working (IHC/P). Does anyone have a source for a CgA that
works in dogs?
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