Hi Blanca, It all depends on what you are trying to stain, how the tissue has been harvested/processed and what are your antibodies. If you are trying to use a primary mouse on mouse tissue, that is always tricky. You will need to probably explore your options with M.O.M kits. Otherwise, you may need to check your primary and secondaries. If you are using a secondary that is rat anti-mouse, you may also need to use an absorbed mouse secondary, to avoid too much non-specific staining.
Kind regards, Ana Ana Maluenda Research Assistant/Laboratory Manager Atherothrombosis and Vascular Biology Laboratory Baker Heart and Diabetes Institute 75 Commercial Road, Melbourne VIC 3004 P (03) 8532 1359 E ana.malue...@baker.edu.au W www.baker.edu.au -----Original Message----- From: Margaryan, Naira <naira.margar...@hsc.wvu.edu> Sent: Friday, 14 June 2019 3:27 AM To: Blanca Lopez <blanca.lo...@utsouthwestern.edu>; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] IHC mouse optomization Biocare Medical has pure ready to use secondary.. Naira -----Original Message----- From: Blanca Lopez <blanca.lo...@utsouthwestern.edu> Sent: Wednesday, June 12, 2019 10:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC mouse optomization Morning, I need a good advice in optimizing Mouse antibodies for research purposes. I use Dako/Agilent products. COX6B2 from Sigma is giving us hard times. Dako kit HRP has a mixture of mouse and rabbit so we think that might be the reason become negative. Is there any tips for optimizing antibodies special for mouse tissue? Or if you can share your best procedures in IHC mouse stains. I can have a different ways and products to try. Any suggestion or opinions count. If you have any website that I can learn more about IHC for research mainly done in Xenograft. Thank you everybody:) Blanca Lopez HT (ASCP)cm Senior Histotechnologist UT Southwestern Medical Center Harold C. Simmons Comprehensive Cancer Center UTSTR Biorepository Tissue Lab 6000 Harry Hines Blvd NB5.102 Dallas, Texas 75390 214-648-7598 blanca.lo...@utsouthwestern.edu<mailto:blanca.lo...@utsouthwestern.edu> ________________________________ UT Southwestern Medical Center The future of medicine, today. Protecting your privacy is important to us. The Baker Heart and Diabetes Institute will handle your information in accordance with the Privacy Act 1988 (Cth) and its Privacy Policy which is available at www.baker.edu.au or on request by contacting priv...@baker.edu.au or by calling 1800 838 498. The Privacy Policy also explains how you can access and correct your personal information, or make a complaint about a breach of the Australian Privacy Principles. bidipp2014.0.1a -- Message protected by MailGuard: e-mail anti-virus, anti-spam and content filtering.http://www.mailguard.com.au/mg _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
