Manahil, we do IF on paraffin sections on occasion when kidney tissue is insufficient (and then only IgA, G, M and K, L) or they want the markers on tissue that is only embedded in paraffin, such as kappa, Lambda for amyloid. We use at a higher concentration than for frozens (1:10 vs 1:100) and use pronase as an antigen retrieval agent.
We use the Leica Bond stainer. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: MANAHIL EL BIREIR via Histonet <histonet@lists.utsouthwestern.edu> Sent: Friday, October 30, 2020 9:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] DIF in paraffin section Hi Histonet: Happy Halloween 🎃 Would like to know if any one shift from DIF in fresh frozen section to embedded paraffin section!? And which IHC auto machine are you using ? Cheers, Have a nice weekend Manahil Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIGaQ&c=iORugZls2LlYyCAZRB3XLg&r=7cy9qXFa73jDX2Iixpjkq1XlWAfHgLLHm33agI_sCKA&m=fffv2XDi6NYbm7KhVGfzGOk-NBqujnyOXTcpDx2NtLQ&s=uoTOe_sXTll_THbnMwr29riXoFMtKY6BI19HZR49gac&e= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
